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Droplet Microfluidics for the ex Vivo Expansion of Human Primary Multiple Myeloma Cells

1
CSIC, Spanish National Research Council, 28006 Madrid, Spain
2
Hospital 12 Octubre, Hematology Department, Research institute i+12, 28040 Madrid, Spain
3
CNIO, Spanish national cancer research Centre, Hematological malignancies research unit, 28029 Madrid, Spain
4
UCM, Complutense University Madrid, Medical faculty, 28040 Madrid, Spain
*
Author to whom correspondence should be addressed.
Micromachines 2020, 11(3), 261; https://doi.org/10.3390/mi11030261
Received: 21 January 2020 / Revised: 21 February 2020 / Accepted: 25 February 2020 / Published: 29 February 2020
(This article belongs to the Special Issue Micromanipulation in Microfluidics)
We previously reported a new approach for micromanipulation and encapsulation of human stem cells using a droplet-based microfluidic device We demonstrated the possibility of encapsulating and culturing difficult-to-preserve primary human hematopoietic stem cells using an engineered double layered bead composed by an inner layer of alginate and an outer layer of puramatrix constructed using a soft technology without the use of any external force. In this work, we use this micro manipulation technique to build a 3D scaffold as a biomimetic model to recapitulate the niche of patient-derived multiple myeloma cells (MM cell) using a multilayered 3D tissue scaffold constructed in a microfluidic device and cultured in 10% FBS culture medium. In the current study, we included the use of this biomimetic model comprising supporting human Mesenchymal stem cells to show the mid-term survival of MM cells in the proposed structures. We found that the generated microniches were suitable for the maintenance of MM cells with and without supporting cells. Additionally, cultured MM cells in droplets were exposed to both Bortezomib and Lenalidomide to test their toxicity in the cultured patient derived cells. Results indicate that the maintained MM cells were consistently responding to the applied medication, opening a wide field of possibilities to use the presented micro device as an ex vivo platform for drug screening. View Full-Text
Keywords: microdroplets; microfluidics; stem cell encapsulation microdroplets; microfluidics; stem cell encapsulation
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Carreras, P.; Gonzalez, I.; Gallardo, M.; Ortiz-Ruiz, A.; Martinez-Lopez, J. Droplet Microfluidics for the ex Vivo Expansion of Human Primary Multiple Myeloma Cells. Micromachines 2020, 11, 261.

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