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Open AccessArticle

Towards PCB-Based Miniaturized Thermocyclers for DNA Amplification

Institute of Nanoscience and Nanotechnology, National Center for Scientific Research “Demokritos”, Patr. Gregoriou E’ and 27 Neapoleos str., 15341 Aghia Paraskevi, Attiki, Greece
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Authors to whom correspondence should be addressed.
Current Address: Department of Food Hygiene & Technology, University of León, 24004 León, Spain.
Current Address: Fast Track Diagnostics S.à.r.l., 29 rue Henri Koch, 4354 Esch-sur-Alzette, Luxembourg.
Micromachines 2020, 11(3), 258; https://doi.org/10.3390/mi11030258
Received: 4 February 2020 / Revised: 24 February 2020 / Accepted: 25 February 2020 / Published: 28 February 2020
(This article belongs to the Special Issue Microfluidics on Printed Circuit Boards)
In recent years, printed circuit board (PCB)-based microfluidics have been explored as a means to achieve standardization, seamless integration, and large-scale manufacturing of microfluidics, thus paving the way for widespread commercialization of developed prototypes. In this work, static micro polymerase chain reaction (microPCR) devices comprising resistive microheaters integrated on PCBs are introduced as miniaturized thermocyclers for efficient DNA amplification. Their performance is compared to that of conventional thermocyclers, in terms of amplification efficiency, power consumption and duration. Exhibiting similar efficiency to conventional thermocyclers, PCB-based miniaturized thermocycling achieves faster DNA amplification, with significantly smaller power consumption. Simulations guide the design of such devices and propose means for further improvement of their performance. View Full-Text
Keywords: printed circuit board (PCB); microfluidics; micro polymerase chain reaction (microPCR); static; multi-well PCR; simulation; genomic Salmonella DNA printed circuit board (PCB); microfluidics; micro polymerase chain reaction (microPCR); static; multi-well PCR; simulation; genomic Salmonella DNA
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MDPI and ACS Style

Kaprou, G.D.; Papadopoulos, V.; Loukas, C.-M.; Kokkoris, G.; Tserepi, A. Towards PCB-Based Miniaturized Thermocyclers for DNA Amplification. Micromachines 2020, 11, 258.

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