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Open AccessArticle

A Quantitative Study of the Secondary Acoustic Radiation Force on Biological Cells during Acoustophoresis

1
Department of Mechanical Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran
2
Department of Applied Physics, Royal Institute of Technology, KTH-AlbaNova, SE-106 91 Stockholm, Sweden
3
Department of Physiology, Applied Physiology Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan 81745, Iran
*
Author to whom correspondence should be addressed.
Micromachines 2020, 11(2), 152; https://doi.org/10.3390/mi11020152
Received: 9 January 2020 / Revised: 28 January 2020 / Accepted: 28 January 2020 / Published: 30 January 2020
(This article belongs to the Special Issue 10th Anniversary of Micromachines)
We investigate cell-particle secondary acoustic radiation forces in a plain ultrasonic standing wave field inside a microfluidic channel. The effect of secondary acoustic radiation forces on biological cells is measured in a location between a pressure node and a pressure anti-node and the result is compared with theory by considering both compressibility and density dependent effects. The secondary acoustic force between motile red blood cells (RBCs) and MCF-7 cells and fixed 20 µm silica beads is investigated in a half-wavelength wide microchannel actuated at 2 MHz ultrasonic frequency. Our study shows that the secondary acoustic force between cells in acoustofluidic devices could play an important role for cell separation, sorting, and trapping purposes. Our results also demonstrate the possibility to isolate individual cells at trapping positions provided by silica beads immobilized and adhered to the microchannel bottom. We conclude that during certain experimental conditions, the secondary acoustic force acting on biological cells can dominate over the primary acoustic radiation force, which could open up for new microscale acoustofluidic methods.
Keywords: acoustophoresis; secondary acoustic radiation forces; cell manipulation acoustophoresis; secondary acoustic radiation forces; cell manipulation
MDPI and ACS Style

Saeidi, D.; Saghafian, M.; Haghjooy Javanmard, S.; Wiklund, M. A Quantitative Study of the Secondary Acoustic Radiation Force on Biological Cells during Acoustophoresis. Micromachines 2020, 11, 152.

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