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Open AccessArticle

An Interphase Microfluidic Culture System for the Study of Ex Vivo Intestinal Tissue

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Univ. Lille, CNRS, ISEN-YNCREA, UMR 8520-IEMN, F-59000 Lille, France
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ISA-YNCREA Hauts de France, F-59000 Lille, France
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Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR 9107-CIIL-Centre d’Infection et d’Immunité de Lille, F-59019 Lille, France
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Laboratoire Ecologie et Biodiversité, Unité de Recherche Smart and Sustainable Cities, Faculté de Gestion Economie et Sciences, Institut Catholique de Lille, F-59800 Lille, France
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Service d’Anatomie et de Cytologie Pathologiques, Groupement des Hôpitaux de l'Université Catholique de Lille, 59000 Lille, France
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CNRS, Univ. Tokyo, UMI 2820 — LIMMS, F-59000 Lille, France
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Plateforme d’Expérimentations et de Hautes Technologies Animales, Institut Pasteur de Lille Lille, 59019 Lille, France
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Délégation à la Recherche Clinique et à l’Innovation, Groupement des Hôpitaux de l'Institut Catholique de Lille (GHICL), Faculté de Médecine et Maïeutique, Université Catholique de Lille, 59800 Lille, France
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Author to whom correspondence should be addressed.
Micromachines 2020, 11(2), 150; https://doi.org/10.3390/mi11020150
Received: 13 November 2019 / Revised: 21 January 2020 / Accepted: 28 January 2020 / Published: 30 January 2020
(This article belongs to the Special Issue Biomedical Microfluidic Devices 2019)
Ex vivo explant culture models offer unique properties to study complex mechanisms underlying tissue growth, renewal, and disease. A major weakness is the short viability depending on the biopsy origin and preparation protocol. We describe an interphase microfluidic culture system to cultivate full thickness murine colon explants which keeps morphological structures of the tissue up to 192 h. The system was composed of a central well on top of a porous membrane supported by a microchannel structure. The microfluidic perfusion allowed bathing the serosal side while preventing immersion of the villi. After eight days, up to 33% of the samples displayed no histological abnormalities. Numerical simulation of the transport of oxygen and glucose provided technical solutions to improve the functionality of the microdevice.
Keywords: organotypic tissue model; intestinal tract model; ex vivo microfluidic tissue culture; long term viability maintenance organotypic tissue model; intestinal tract model; ex vivo microfluidic tissue culture; long term viability maintenance
MDPI and ACS Style

Baydoun, M.; Treizeibré, A.; Follet, J.; Vanneste, S.B.; Creusy, C.; Dercourt, L.; Delaire, B.; Mouray, A.; Viscogliosi, E.; Certad, G.; Senez, V. An Interphase Microfluidic Culture System for the Study of Ex Vivo Intestinal Tissue. Micromachines 2020, 11, 150.

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