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Open AccessFeature PaperArticle

A Fully Integrated In Vitro Diagnostic Microsystem for Pathogen Detection Developed Using a “3D Extensible” Microfluidic Design Paradigm

by Zhi Geng 1, Yin Gu 1,2, Shanglin Li 1,2, Baobao Lin 1 and Peng Liu 1,*
1
Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China
2
FengteBio Corporation, Beijing 100079, China
*
Author to whom correspondence should be addressed.
Micromachines 2019, 10(12), 873; https://doi.org/10.3390/mi10120873
Received: 25 November 2019 / Revised: 4 December 2019 / Accepted: 10 December 2019 / Published: 12 December 2019
(This article belongs to the Section B:Biology and Biomedicine)
Microfluidics is facing critical challenges in the quest of miniaturizing, integrating, and automating in vitro diagnostics, including the increasing complexity of assays, the gap between the macroscale world and the microscale devices, and the diverse throughput demands in various clinical settings. Here, a “3D extensible” microfluidic design paradigm that consists of a set of basic structures and unit operations was developed for constructing any application-specific assay. Four basic structures—check valve (in), check valve (out), double-check valve (in and out), and on–off valve—were designed to mimic basic acts in biochemical assays. By combining these structures linearly, a series of unit operations can be readily formed. We then proposed a “3D extensible” architecture to fulfill the needs of the function integration, the adaptive “world-to-chip” interface, and the adjustable throughput in the X, Y, and Z directions, respectively. To verify this design paradigm, we developed a fully integrated loop-mediated isothermal amplification microsystem that can directly accept swab samples and detect Chlamydia trachomatis automatically with a sensitivity one order higher than that of the conventional kit. This demonstration validated the feasibility of using this paradigm to develop integrated and automated microsystems in a less risky and more consistent manner. View Full-Text
Keywords: in vitro diagnostics; microfluidics; full integration; lab-on-a-chip; pathogen detection in vitro diagnostics; microfluidics; full integration; lab-on-a-chip; pathogen detection
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Geng, Z.; Gu, Y.; Li, S.; Lin, B.; Liu, P. A Fully Integrated In Vitro Diagnostic Microsystem for Pathogen Detection Developed Using a “3D Extensible” Microfluidic Design Paradigm. Micromachines 2019, 10, 873.

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