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Open AccessArticle

Microfluidic Time-Delay Valve Mechanism on Paper-Based Devices for Automated Competitive ELISA

1
Department of Bio-Industrial Mechatronics Engineering, National Taiwan University, Taipei 10016, Taiwan
2
Department of Entomology, National Taiwan University, Taipei 10016, Taiwan
*
Author to whom correspondence should be addressed.
Micromachines 2019, 10(12), 837; https://doi.org/10.3390/mi10120837
Received: 12 November 2019 / Revised: 25 November 2019 / Accepted: 28 November 2019 / Published: 30 November 2019
(This article belongs to the Special Issue Next Generation Paper-based Devices for Analytical Applications)
Paper-based technologies have been drawing increasing attentions in the biosensor field due to their economical, ecofriendly, and easy-to-fabricate features. In this paper, we present a time-delay valve mechanism to automate a series of procedures for conducting competitive enzyme-linked immunosorbent assay (ELISA) on a paper-based device. The mechanism employs a controllable time-delay valve, which has surfactants to dissolve the hydrophobic barriers, in a fluid pathway. The valves can regulate the liquid and sequentially deliver the sample flow for automating ELISA procedures in microchannels. Competitive ELISA is achieved in a single step once the sample, or small molecule pesticide (e.g., Imidacloprid), is applied onto the paper-based device with a comparable sensitivity to plate-based competitive ELISA. The results further demonstrate the appositeness of using paper-based devices with the valve designs for on-the-go ELISA detection in agriculture and biomedical applications. View Full-Text
Keywords: microfluidic valve; competitive enzyme-linked immunosorbent assay (ELISA); pesticide residue microfluidic valve; competitive enzyme-linked immunosorbent assay (ELISA); pesticide residue
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MDPI and ACS Style

Lai, Y.-T.; Tsai, C.-H.; Hsu, J.-C.; Lu, Y.-W. Microfluidic Time-Delay Valve Mechanism on Paper-Based Devices for Automated Competitive ELISA. Micromachines 2019, 10, 837.

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