Next Article in Journal
Visual Servo Control System of a Piezoelectric2-Degree-of-Freedom Nano-Stepping Motor
Previous Article in Journal
A Microfluidic Rotational Motor Driven by Circular Vibrations
Previous Article in Special Issue
Microneedle Patterning of 3D Nonplanar Surfaces on Implantable Medical Devices Using Soft Lithography
Open AccessArticle

A Micropatterning Strategy to Study Nuclear Mechanotransduction in Cells

1
Department of Chemistry, University of Southern California, Los Angeles, CA 90089, USA
2
Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089, USA
3
Department of Physics and Astronomy, University of Southern California, Los Angeles, CA 90089, USA
*
Author to whom correspondence should be addressed.
Micromachines 2019, 10(12), 810; https://doi.org/10.3390/mi10120810
Received: 28 October 2019 / Revised: 22 November 2019 / Accepted: 22 November 2019 / Published: 24 November 2019
(This article belongs to the Special Issue New Approaches to Micropatterning)
Micropatterning techniques have been widely used in biology, particularly in studies involving cell adhesion and proliferation on different substrates. Cell micropatterning approaches are also increasingly employed as in vitro tools to investigate intracellular mechanotransduction processes. In this report, we examined how modulating cellular shapes on two-dimensional rectangular fibronectin micropatterns of different widths influences nuclear mechanotransduction mediated by emerin, a nuclear envelope protein implicated in Emery–Dreifuss muscular dystrophy (EDMD). Fibronectin microcontact printing was tested onto glass coverslips functionalized with three different silane reagents (hexamethyldisilazane (HMDS), (3-Aminopropyl)triethoxysilane (APTES) and (3-Glycidyloxypropyl)trimethoxysilane (GPTMS)) using a vapor-phase deposition method. We observed that HMDS provides the most reliable printing surface for cell micropatterning, notably because it forms a hydrophobic organosilane monolayer that favors the retainment of surface antifouling agents on the coverslips. We showed that, under specific mechanical cues, emerin-null human skin fibroblasts display a significantly more deformed nucleus than skin fibroblasts expressing wild type emerin, indicating that emerin plays a crucial role in nuclear adaptability to mechanical stresses. We further showed that proper nuclear responses to forces involve a significant relocation of emerin from the inner nuclear envelope towards the outer nuclear envelope and the endoplasmic reticulum membrane network. Cell micropatterning by fibronectin microcontact printing directly on HMDS-treated glass represents a simple approach to apply steady-state biophysical cues to cells and study their specific mechanobiology responses in vitro. View Full-Text
Keywords: surface silanization; cell micropatterning; emerin; lamin; Emery–Dreifuss muscular dystrophy (EDMD); nuclear shape index; mechanotransduction surface silanization; cell micropatterning; emerin; lamin; Emery–Dreifuss muscular dystrophy (EDMD); nuclear shape index; mechanotransduction
Show Figures

Figure 1

MDPI and ACS Style

Bautista, M.; Fernandez, A.; Pinaud, F. A Micropatterning Strategy to Study Nuclear Mechanotransduction in Cells. Micromachines 2019, 10, 810.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop