Optimization of Islet Microencapsulation with Thin Polymer Membranes for Long-Term Stability
by
Shota Toda 1, Artin Fattah 2, Kenta Asawa 3, Naoko Nakamura 1
, Kristina N. Ekdahl 2,4, Bo Nilsson 2 and Yuji Teramura 2,3,*
Shota Toda 1, Artin Fattah 2, Kenta Asawa 3, Naoko Nakamura 1, Kristina N. Ekdahl 2,4, Bo Nilsson 2 and Yuji Teramura 2,3,*
1
Department of Bioscience and Engineering, College of Systems Engineering and Science, Shibaura Institute of Technology, Saitama 337-8570, Japan
2
Department of Immunology, Genetics and Pathology (IGP), Uppsala University, Dag Hammarskjölds väg 20, SE-751 85 Uppsala, Sweden
3
Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
4
Linnaeus Center of Biomaterials Chemistry, Linnaeus University, SE-391 82 Kalmar, Sweden
*
Author to whom correspondence should be addressed.
Micromachines 2019, 10(11), 755; https://doi.org/10.3390/mi10110755
Received: 5 October 2019 / Revised: 30 October 2019 / Accepted: 4 November 2019 / Published: 6 November 2019
(This article belongs to the Special Issue Micro/Nano Devices in Biological Medicine)
Microencapsulation of islets can protect against immune reactions from the host immune system after transplantation. However, sufficient numbers of islets cannot be transplanted due to the increase of the size and total volume. Therefore, thin and stable polymer membranes are required for the microencapsulation. Here, we undertook the cell microencapsulation using poly(ethylene glycol)-conjugated phospholipid (PEG-lipid) and layer-by-layer membrane of multiple-arm PEG. In order to examine the membrane stability, we used different molecular weights of 4-arm PEG (10k, 20k and 40k)-Mal to examine the influence on the polymer membrane stability. We found that the polymer membrane made of 4-arm PEG(40k)-Mal showed the highest stability on the cell surface. Also, the polymer membrane did not disturb the insulin secretion from beta cells.
View Full-Text
Keywords:
microencapsulation; bioartificial pancreas; islet transplantation; polyethylene glycol-lipid (PEG-lipid); cell surface modification
▼
Show Figures
Graphical abstract
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
MDPI and ACS Style
Toda, S.; Fattah, A.; Asawa, K.; Nakamura, N.; N. Ekdahl, K.; Nilsson, B.; Teramura, Y. Optimization of Islet Microencapsulation with Thin Polymer Membranes for Long-Term Stability. Micromachines 2019, 10, 755.
Show more citation formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.
