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Toxins 2017, 9(9), 270; https://doi.org/10.3390/toxins9090270

Spread of Tst–Positive Staphylococcus aureus Strains Belonging to ST30 Clone among Patients and Healthcare Workers in Two Intensive Care Units

1
Division of Infectious Diseases, School of Medicine, University of Patras, 26504 Rion-Patras, Greece
2
Department of Microbiology, School of Medicine, University of Patras, 26504 Rion-Patras, Greece
3
National Reference Laboratory for Staphylococci, School of Medicine, University of Patras, 26504 Rion-Patras, Greece
4
Department of Anaesthesiology and Intensive Care Medicine, University of Patras, 26504 Rion-Patras, Greece
5
Intensive Care Unit, Saint Andrew’s General Hospital, 26335 Patras, Greece
6
Department of Microbiology, School of Medicine, University of Thessaly, Biopolis, 41222 Larissa, Greece
*
Author to whom correspondence should be addressed.
Academic Editor: Andreas Rummel
Received: 9 August 2017 / Revised: 24 August 2017 / Accepted: 31 August 2017 / Published: 4 September 2017
(This article belongs to the Collection Staphylococcus aureus Toxins)
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Abstract

Staphylococcus aureus is a major cause of infections. Toxic shock syndrome toxin (TSST-1) and Panton–Valentine leukocidin (PVL) are associated with severe clinical syndromes. S. aureus colonizing isolates recovered from healthcare workers and patients in the intensive care unit (ICU) of a university hospital comprising Group A were compared with those from adult non-ICU carriers (Group B). mecA, lukS/lukF-PV (Panton–Valentine leukocidin, PVL), and tst (toxic shock syndrome toxin) gene carriage was detected by PCR. Clones were identified in all methicillin-resistant S. aureus (MRSA) and toxin-positive methicillin-susceptible strains (MSSA) by pulsed-field gel electrophoresis (PFGE), agr groups, and multi locus sequencing typing (MLST). Group A included 90 S. aureus isolates, whereas Group B 53. PVL was more frequently found among MRSA vs. MSSA (p < 0.001) and in strains of Group B as compared to Group A (p < 0.001), consistent with the spread of ST80-IV. Higher incidence of tst gene carriage was identified among MSSA vs. MRSA (P 0.005) belonging mainly to ST30, and Group A vs. Group B (P 0.002). The wide dissemination of ST80-IV mainly in the community is responsible for a high percentage of PVL-positive MRSA, while silent spread of tst-positive S. aureus clones among ICU patients and personnel poses a threat of hospital transmission and possible severe infections. View Full-Text
Keywords: carriage; MRSA; critically ill patients; toxic shock syndrome toxin (TSST-1); Panton–Valentine leukocidin (PVL); MLST; daptomycin; linezolid; vancomycin carriage; MRSA; critically ill patients; toxic shock syndrome toxin (TSST-1); Panton–Valentine leukocidin (PVL); MLST; daptomycin; linezolid; vancomycin
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Papadimitriou-Olivgeris, M.; Drougka, E.; Fligou, F.; Dodou, V.; Kolonitsiou, F.; Filos, K.S.; Anastassiou, E.D.; Petinaki, E.; Marangos, M.; Spiliopoulou, I. Spread of Tst–Positive Staphylococcus aureus Strains Belonging to ST30 Clone among Patients and Healthcare Workers in Two Intensive Care Units. Toxins 2017, 9, 270.

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