Heterologous Expression, Purification and Immunoreactivity of the Antigen 5 from Polybia paulista Wasp Venom
Abstract
:1. Introduction
2. Results
2.1. Recombinant Poly p 5 Coding Sequence
2.2. Heterologous Expression and Purification of rPoly p 5
2.3. Recombinant Allergen Purification
2.4. IgE-Mediated Immunodetection
3. Discussion
4. Concluding Remarks
5. Materials and Methods
5.1. Sera from Allergic Patients
5.2. Polybia paulista Venom Collection
5.3. Poly p 5 cDNA Cloning and Sequencing
5.4. Poly p 5 Recombinant Expression
5.5. Purification of the Recombinant and Native Poly p 5
5.6. SDS-PAGE and Protein Quantification
5.7. Anti-His Immunodetection and Immunoblotting Analyses
5.8. Molecular Modelling
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
Poly p 5 | Antigen 5 from Polybia paulista venom |
Poly p 1 | phospholipase A1 from Polybia paulista venom |
CCDs | Cross-reactive carbohydrate determinants |
HVA | Hymenoptera venom allergy |
HBV | Honeybee venom |
CRD | Component resolved diagnosis |
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Bazon, M.L.; Perez-Riverol, A.; Dos Santos-Pinto, J.R.A.; Fernandes, L.G.R.; Lasa, A.M.; Justo-Jacomini, D.L.; Palma, M.S.; Zollner, R.D.L.; Brochetto-Braga, M.R. Heterologous Expression, Purification and Immunoreactivity of the Antigen 5 from Polybia paulista Wasp Venom. Toxins 2017, 9, 259. https://doi.org/10.3390/toxins9090259
Bazon ML, Perez-Riverol A, Dos Santos-Pinto JRA, Fernandes LGR, Lasa AM, Justo-Jacomini DL, Palma MS, Zollner RDL, Brochetto-Braga MR. Heterologous Expression, Purification and Immunoreactivity of the Antigen 5 from Polybia paulista Wasp Venom. Toxins. 2017; 9(9):259. https://doi.org/10.3390/toxins9090259
Chicago/Turabian StyleBazon, Murilo Luiz, Amilcar Perez-Riverol, José Roberto Aparecido Dos Santos-Pinto, Luis Gustavo Romani Fernandes, Alexis Musacchio Lasa, Débora Laís Justo-Jacomini, Mario Sergio Palma, Ricardo De Lima Zollner, and Márcia Regina Brochetto-Braga. 2017. "Heterologous Expression, Purification and Immunoreactivity of the Antigen 5 from Polybia paulista Wasp Venom" Toxins 9, no. 9: 259. https://doi.org/10.3390/toxins9090259
APA StyleBazon, M. L., Perez-Riverol, A., Dos Santos-Pinto, J. R. A., Fernandes, L. G. R., Lasa, A. M., Justo-Jacomini, D. L., Palma, M. S., Zollner, R. D. L., & Brochetto-Braga, M. R. (2017). Heterologous Expression, Purification and Immunoreactivity of the Antigen 5 from Polybia paulista Wasp Venom. Toxins, 9(9), 259. https://doi.org/10.3390/toxins9090259