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Microbial Detoxification of Deoxynivalenol (DON), Assessed via a Lemna minor L. Bioassay, through Biotransformation to 3-epi-DON and 3-epi-DOM-1
by
Ilse Vanhoutte 1, Laura De Mets 1, Marthe De Boevre 2
, Valdet Uka 2, José Diana Di Mavungu 2, Sarah De Saeger 2, Leen De Gelder 1,*,† and Kris Audenaert 3,†
Ilse Vanhoutte 1, Laura De Mets 1, Marthe De Boevre 2, Valdet Uka 2, José Diana Di Mavungu 2, Sarah De Saeger 2, Leen De Gelder 1,*,† and Kris Audenaert 3,†
1
Laboratory of Environmental Biotechnology, Department of Applied Biosciences, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium
2
Laboratory of Food Analysis, Department of Bioanalysis, Faculty of Pharmaceutical Sciences, Ghent University, 9000 Ghent, Belgium
3
Laboratory of Applied Mycology and Phenomics, Department of Applied Biosciences, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Academic Editor: Massimo Reverberi
Toxins 2017, 9(2), 63; https://doi.org/10.3390/toxins9020063
Received: 31 August 2016 / Revised: 31 January 2017 / Accepted: 4 February 2017 / Published: 13 February 2017
(This article belongs to the Special Issue Selected Papers from the 5th International Symposium on Mycotoxins and Toxigenic Moulds: Challenges and Perspectives)
Mycotoxins are toxic metabolites produced by fungi. To mitigate mycotoxins in food or feed, biotransformation is an emerging technology in which microorganisms degrade toxins into non-toxic metabolites. To monitor deoxynivalenol (DON) biotransformation, analytical tools such as ELISA and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) are typically used. However, these techniques do not give a decisive answer about the remaining toxicity of possible biotransformation products. Hence, a bioassay using Lemna minor L. was developed. A dose–response analysis revealed significant inhibition in the growth of L. minor exposed to DON concentrations of 0.25 mg/L and higher. Concentrations above 1 mg/L were lethal for the plant. This bioassay is far more sensitive than previously described systems. The bioassay was implemented to screen microbial enrichment cultures, originating from rumen fluid, soil, digestate and activated sludge, on their biotransformation and detoxification capability of DON. The enrichment cultures originating from soil and activated sludge were capable of detoxifying and degrading 5 and 50 mg/L DON. In addition, the metabolites 3-epi-DON and the epimer of de-epoxy-DON (3-epi-DOM-1) were found as biotransformation products of both consortia. Our work provides a new valuable tool to screen microbial cultures for their detoxification capacity.
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Keywords:
deoxynivalenol (DON); Lemna minor; bioassay; biotransformation; detoxification; 3-epi-DON; 3-epi-de-epoxy-DON (3-epi-DOM-1)
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MDPI and ACS Style
Vanhoutte, I.; De Mets, L.; De Boevre, M.; Uka, V.; Di Mavungu, J.D.; De Saeger, S.; De Gelder, L.; Audenaert, K. Microbial Detoxification of Deoxynivalenol (DON), Assessed via a Lemna minor L. Bioassay, through Biotransformation to 3-epi-DON and 3-epi-DOM-1. Toxins 2017, 9, 63.
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