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Open AccessFeature PaperArticle

Comparison of Enzyme-Linked Immunosorbent Assay, Surface Plasmon Resonance and Biolayer Interferometry for Screening of Deoxynivalenol in Wheat and Wheat Dust

Mycotoxin Prevention and Applied Microbiology Research Unit, United States Department of Agriculture-Agricultural Research Service-National Center for Agricultural Utilization Research (USDA-ARS-NCAUR), 1815 N. University Street, Peoria, IL 61604, USA
School of Biotechnology, National Centre for Sensor Research and Biomedical Diagnostic Institute, Dublin City University, Dublin 9, Ireland
Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310029, China
Department of Applied Biosciences, Faculty of Bioscience Engineering, Ghent University, Valentin Vaerwyckweg 1, 9000 Ghent, Belgium
Department of Bioanalysis, Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium
Author to whom correspondence should be addressed.
Academic Editors: Michelangelo Pascale and Maria C. DeRosa
Toxins 2016, 8(4), 103;
Received: 5 February 2016 / Revised: 28 March 2016 / Accepted: 30 March 2016 / Published: 11 April 2016
(This article belongs to the Collection Biorecognition Assays for Mycotoxins)
PDF [922 KB, uploaded 11 April 2016]


A sample preparation method was developed for the screening of deoxynivalenol (DON) in wheat and wheat dust. Extraction was carried out with water and was successful due to the polar character of DON. For detection, an enzyme-linked immunosorbent assay (ELISA) was compared to the sensor-based techniques of surface plasmon resonance (SPR) and biolayer interferometry (BLI) in terms of sensitivity, affinity and matrix effect. The matrix effects from wheat and wheat dust using SPR were too high to further use this screenings method. The preferred ELISA and BLI methods were validated according to the criteria established in Commission Regulation 519/2014/EC and Commission Decision 2002/657/EC. A small survey was executed on 16 wheat lots and their corresponding dust samples using the validated ELISA method. A linear correlation (r = 0.889) was found for the DON concentration in dust versus the DON concentration in wheat (LOD wheat: 233 μg/kg, LOD wheat dust: 458 μg/kg). View Full-Text
Keywords: deoxynivalenol; monoclonal antibodies; immunosensor; wheat; wheat dust deoxynivalenol; monoclonal antibodies; immunosensor; wheat; wheat dust

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Sanders, M.; McPartlin, D.; Moran, K.; Guo, Y.; Eeckhout, M.; O’Kennedy, R.; De Saeger, S.; Maragos, C. Comparison of Enzyme-Linked Immunosorbent Assay, Surface Plasmon Resonance and Biolayer Interferometry for Screening of Deoxynivalenol in Wheat and Wheat Dust. Toxins 2016, 8, 103.

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