Next Article in Journal
Comparison of Enzyme-Linked Immunosorbent Assay, Surface Plasmon Resonance and Biolayer Interferometry for Screening of Deoxynivalenol in Wheat and Wheat Dust
Next Article in Special Issue
Quantitative Proteomic Analysis of Venoms from Russian Vipers of Pelias Group: Phospholipases A2 are the Main Venom Components
Previous Article in Journal
Detection and Quantification of ADP-Ribosylated RhoA/B by Monoclonal Antibody
Previous Article in Special Issue
Characterization and Recombinant Expression of Terebrid Venom Peptide from Terebra guttata
Open AccessArticle

Tentacle Transcriptome and Venom Proteome of the Pacific Sea Nettle, Chrysaora fuscescens (Cnidaria: Scyphozoa)

by Dalia Ponce 1,†, Diane L. Brinkman 2,*,†, Jeremy Potriquet 3 and Jason Mulvenna 3,4,*,†
Australian Australian Venom Research Unit, Department of Pharmacology and Therapeutics, University of Melbourne, Melbourne, Victoria 3010, Australia
Australian Institute of Marine Science, Townsville, Queensland 4810, Australia
Queensland Institute of Medical Research (QIMR) Berghofer Medical Research Institute, Infectious Diseases Program, Brisbane, Queensland 4006, Australia
School of Biomedical Sciences, The University of Queensland, Brisbane, Queensland 4072, Australia
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Ronald A. Jenner
Toxins 2016, 8(4), 102;
Received: 13 January 2016 / Revised: 7 March 2016 / Accepted: 22 March 2016 / Published: 5 April 2016
(This article belongs to the Special Issue Venomics, Venom Proteomics and Venom Transcriptomics)
Jellyfish venoms are rich sources of toxins designed to capture prey or deter predators, but they can also elicit harmful effects in humans. In this study, an integrated transcriptomic and proteomic approach was used to identify putative toxins and their potential role in the venom of the scyphozoan jellyfish Chrysaora fuscescens. A de novo tentacle transcriptome, containing more than 23,000 contigs, was constructed and used in proteomic analysis of C. fuscescens venom to identify potential toxins. From a total of 163 proteins identified in the venom proteome, 27 were classified as putative toxins and grouped into six protein families: proteinases, venom allergens, C-type lectins, pore-forming toxins, glycoside hydrolases and enzyme inhibitors. Other putative toxins identified in the transcriptome, but not the proteome, included additional proteinases as well as lipases and deoxyribonucleases. Sequence analysis also revealed the presence of ShKT domains in two putative venom proteins from the proteome and an additional 15 from the transcriptome, suggesting potential ion channel blockade or modulatory activities. Comparison of these potential toxins to those from other cnidarians provided insight into their possible roles in C. fuscescens venom and an overview of the diversity of potential toxin families in cnidarian venoms. View Full-Text
Keywords: Jellyfish; Chrysaora; venom; transcriptome; proteome; toxin; nematocyst Jellyfish; Chrysaora; venom; transcriptome; proteome; toxin; nematocyst
Show Figures

Figure 1

MDPI and ACS Style

Ponce, D.; Brinkman, D.L.; Potriquet, J.; Mulvenna, J. Tentacle Transcriptome and Venom Proteome of the Pacific Sea Nettle, Chrysaora fuscescens (Cnidaria: Scyphozoa). Toxins 2016, 8, 102.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop