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Toxins 2016, 8(3), 66;

DNA Aptamers against Taiwan Banded Krait α-Bungarotoxin Recognize Taiwan Cobra Cardiotoxins

Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung 804, Taiwan
Department of Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan
Author to whom correspondence should be addressed.
Academic Editor: Nicholas R. Casewell
Received: 31 January 2016 / Revised: 29 February 2016 / Accepted: 29 February 2016 / Published: 5 March 2016
(This article belongs to the Section Animal Venoms)
Full-Text   |   PDF [2015 KB, uploaded 5 March 2016]   |  


Bungarus multicinctus α-bungarotoxin (α-Bgt) and Naja atra cardiotoxins (CTXs) share a common structural scaffold, and their tertiary structures adopt three-fingered loop motifs. Four DNA aptamers against α-Bgt have been reported previously. Given that the binding of aptamers with targeted proteins depends on structural complementarity, in this study, we investigated whether DNA aptamers against α-Bgt could also recognize CTXs. It was found that N. atra cardiotoxin 3 (CTX3) reduced the electrophoretic mobility of aptamers against α-Bgt. Analysis of the changes in the fluorescence intensity of carboxyfluorescein-labeled aptamers upon binding toxin molecules revealed that CTX3 and α-Bgt could bind the tested aptamers. Moreover, the aptamers inhibited the membrane-damaging activity and cytotoxicity of CTX3. In addition to CTX3, other N. atra CTX isotoxins also bound to the aptamer against α-Bgt. Taken together, our data indicate that aptamers against α-Bgt show cross-reactivity with CTXs. The findings that aptamers against α-Bgt also suppress the biological activities of CTX3 highlight the potential utility of aptamers in regard to the broad inhibition of snake venom three-fingered proteins. View Full-Text
Keywords: aptamer; α-bungarotoxin; cardiotoxins; membrane-damaging activity; cytotoxicity aptamer; α-bungarotoxin; cardiotoxins; membrane-damaging activity; cytotoxicity

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Chen, Y.-J.; Tsai, C.-Y.; Hu, W.-P.; Chang, L.-S. DNA Aptamers against Taiwan Banded Krait α-Bungarotoxin Recognize Taiwan Cobra Cardiotoxins. Toxins 2016, 8, 66.

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