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Open AccessArticle

Microcystin-Bound Protein Patterns in Different Cultures of Microcystis aeruginosa and Field Samples

by Nian Wei 1,2, Lili Hu 1,2, Lirong Song 1,* and Nanqin Gan 1,*
1
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
2
University of Chinese Academy of Sciences, Beijing 100049, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Luis Botana
Toxins 2016, 8(10), 293; https://doi.org/10.3390/toxins8100293
Received: 6 September 2016 / Revised: 6 October 2016 / Accepted: 8 October 2016 / Published: 12 October 2016
(This article belongs to the Collection Marine and Freshwater Toxins)
Micocystin (MC) exists in Microcystis cells in two different forms, free and protein-bound. We examined the dynamic change in extracellular free MCs, intracellular free MCs and protein-bound MCs in both batch cultures and semi-continuous cultures, using high performance liquid chromatography and Western blot. The results showed that the free MC per cell remained constant, while the quantity of protein-bound MCs increased with the growth of Microcystis cells in both kinds of culture. Significant changes in the dominant MC-bound proteins occurred in the late exponential growth phase of batch cultures, while the dominant MC-bound proteins in semi-continuous cultures remained the same. In field samples collected at different months in Lake Taihu, the dominant MC-bound proteins were shown to be similar, but the amount of protein-bound MC varied and correlated with the intracellular MC content. We identified MC-bound proteins by two-dimensional electrophoresis immunoblots and mass spectrometry. The 60 kDa chaperonin GroEL was a prominent MC-bound protein. Three essential glycolytic enzymes and ATP synthase alpha subunit were also major targets of MC-binding, which might contribute to sustained growth in semi-continuous culture. Our results indicate that protein-bound MC may be important for sustaining growth and adaptation of Microcystis sp. View Full-Text
Keywords: microcystin; microcystin-bound protein; batch culture; semi-continuous culture; Microcystis aeruginosa; Lake Taihu microcystin; microcystin-bound protein; batch culture; semi-continuous culture; Microcystis aeruginosa; Lake Taihu
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Wei, N.; Hu, L.; Song, L.; Gan, N. Microcystin-Bound Protein Patterns in Different Cultures of Microcystis aeruginosa and Field Samples. Toxins 2016, 8, 293.

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