Next Article in Journal
Identification and Quantification of a Toxigenic and Non-Toxigenic Aspergillus flavus Strain in Contaminated Maize Using Quantitative Real-Time PCR
Next Article in Special Issue
Rapid Detection of Escherichia coli O157 and Shiga Toxins by Lateral Flow Immunoassays
Previous Article in Journal
Alkaloids from Veratrum taliense Exert Cardiovascular Toxic Effects via Cardiac Sodium Channel Subtype 1.5
Previous Article in Special Issue
Use of Monoclonal Antibodies in the Sensitive Detection and Neutralization of Botulinum Neurotoxin Serotype B
Open AccessFeature PaperArticle

Rapid Microfluidic Assay for the Detection of Botulinum Neurotoxin in Animal Sera

1
Produce Safety & Microbiology Research Unit, United States Department of Agriculture, Agricultural Research Service, 800 Buchanan St, Albany, CA 94710, USA
2
Foodborne Toxin Detection and Prevention Unit, United States Department of Agriculture, Agricultural Research Service, 800 Buchanan St, Albany, CA 94710, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Daniel Gillet
Toxins 2016, 8(1), 13; https://doi.org/10.3390/toxins8010013
Received: 9 December 2015 / Revised: 23 December 2015 / Accepted: 25 December 2015 / Published: 4 January 2016
(This article belongs to the Collection Rapid Detection of Bacterial Toxins)
Potent Botulinum neurotoxins (BoNTs) represent a threat to public health and safety. Botulism is a disease caused by BoNT intoxication that results in muscle paralysis that can be fatal. Sensitive assays capable of detecting BoNTs from different substrates and settings are essential to limit foodborne contamination and morbidity. In this report, we describe a rapid 96-well microfluidic double sandwich immunoassay for the sensitive detection of BoNT-A from animal sera. This BoNT microfluidic assay requires only 5 μL of serum, provides results in 75 min using a standard fluorescence microplate reader and generates minimal hazardous waste. The assay has a <30 pg·mL−1 limit of detection (LOD) of BoNT-A from spiked human serum. This sensitive microfluidic BoNT-A assay offers a fast and simplified workflow suitable for the detection of BoNT-A from serum samples of limited volume in most laboratory settings. View Full-Text
Keywords: toxin; botulinum neurotoxin (BoNT); botulism; microfluidic; immunoassay; rapid detection; animal serum; diagnostic toxin; botulinum neurotoxin (BoNT); botulism; microfluidic; immunoassay; rapid detection; animal serum; diagnostic
Show Figures

Figure 1

MDPI and ACS Style

Babrak, L.; Lin, A.; Stanker, L.H.; McGarvey, J.; Hnasko, R. Rapid Microfluidic Assay for the Detection of Botulinum Neurotoxin in Animal Sera. Toxins 2016, 8, 13. https://doi.org/10.3390/toxins8010013

AMA Style

Babrak L, Lin A, Stanker LH, McGarvey J, Hnasko R. Rapid Microfluidic Assay for the Detection of Botulinum Neurotoxin in Animal Sera. Toxins. 2016; 8(1):13. https://doi.org/10.3390/toxins8010013

Chicago/Turabian Style

Babrak, Lmar; Lin, Alice; Stanker, Larry H.; McGarvey, Jeffery; Hnasko, Robert. 2016. "Rapid Microfluidic Assay for the Detection of Botulinum Neurotoxin in Animal Sera" Toxins 8, no. 1: 13. https://doi.org/10.3390/toxins8010013

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop