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Open AccessArticle

Separation of Binding Protein of Celangulin V from the Midgut of Mythimna separata Walker by Affinity Chromatography

by Lina Lu 1,†, Zhijun Qi 1,†, Jiwen Zhang 2 and Wenjun Wu 1,*
1
Institute of Pesticide Science, College of Plant Protection, Northwest A & F University, Yangling 712100, Shaanxi, China
2
College of Science, Northwest A & F University, Yangling 712100, Shaanxi, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Tzi Bun Ng
Toxins 2015, 7(5), 1738-1748; https://doi.org/10.3390/toxins7051738
Received: 5 March 2015 / Revised: 23 April 2015 / Accepted: 28 April 2015 / Published: 19 May 2015
Celangulin V, an insecticidal compound isolated from the root bark of Chinese bittersweet, can affect the digestive system of insects. However, the mechanism of how Celangulin V induces a series of symptoms is still unknown. In this study, affinity chromatography was conducted through coupling of Celangulin V-6-aminoacetic acid ester to the CNBr-activated Sepharose 4B. SDS-PAGE was used to analyze the collected fraction eluted by Celangulin V. Eight binding proteins (Zinc finger protein, Thioredoxin peroxidase (TPx), Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), SUMO E3 ligase RanBP2, Transmembrane protein 1, Actin, APN and V-ATPase) were obtained and identified by LC/Q-TOF-MS from the midgut of Mythimna separata larvae. The potential of these proteins to serve as target proteins involved in the insecticidal activity of Celangulin V is discussed. View Full-Text
Keywords: Celangulin V; binding protein; affinity chromatography; Mythimna separata Walker Celangulin V; binding protein; affinity chromatography; Mythimna separata Walker
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Lu, L.; Qi, Z.; Zhang, J.; Wu, W. Separation of Binding Protein of Celangulin V from the Midgut of Mythimna separata Walker by Affinity Chromatography. Toxins 2015, 7, 1738-1748.

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