Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold Nanoparticles
Abstract
:1. Introduction
2. Experimental
2.1. Reagents and Apparatus
2.2. Preparation of AuNPs
2.3. Colorimetric Detection of Ochratoxin A
3. Results and Discussion
3.1. Principles of the Colorimetric Method for Ochratoxin A Detection
3.2. Optimization of Experimental Conditions
3.3. Detection of Ochratoxin A with the Label-Free Aptamer-Based Assay
Method | Recognition Part | Limits of detection | Time | References |
---|---|---|---|---|
TLC a | ND f | 0.05–0.93 ng·mL−1 | >2 h | [6] |
HPLC-FLD b | ND f | 0.05–0.41 ng·mL−1 | >2 h | [7,34] |
LC-MS/MS c | ND f | 0.01–0.18 ng·mL−1 | >2 h | [35] |
ELISA d | Antibody | 0.2–5.0 ng·mL−1 | 110 min | [10] |
FPIA e | Antibody | 0.7 ng·mL−1 | 10 min | [36,37] |
Antibody | 0.8 μg/kg | 20 min | [36] | |
Aptamer | 2–5 ng·mL−1 | 45 min | [38] | |
Aptasensor based on electrochemical assay | Aptamer | 0.02 pg·mL−1–0.07 ng·mL−1 | 30 min–1 h | [39,40] |
Aptasensor based on fluorescence assay | Aptamer | 3.6 ng·mL−1 | 30 min–1 h | [41] |
Aptamer-based assay based on AuNPs and poly diallyldimethylammonium chloride | Aptamer | 0.009 ng·mL−1 | 15 min | This work |
3.4. Detection Specificity
3.5. Practicality of Ochratoxin A Detection in Liquor Samples
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
- Turcotte, A.M.; Scott, P.M.; Tague, B. Analysis of cocoa products for ochratoxin A and aflatoxins. Mycotoxin Res. 2013, 29, 193–201. [Google Scholar] [CrossRef] [PubMed]
- Lv, X.C.; Huang, Z.Q.; Zhang, W.; Rao, P.F.; Ni, L. Identification and characterization of filamentous fungi isolated from fermentation starters for Hong Qu glutinous rice wine brewing. J. Gen. Appl. Microbiol. 2012, 58, 33–42. [Google Scholar] [CrossRef] [PubMed]
- Coronel, M.B.; Marin, S.; Cano-Sancho, G.; Ramos, A.J.; Sanchis, V. Exposure assessment to ochratoxin A in Catalonia (Spain) based on the consumption of cereals, nuts, coffee, wine, and beer. Food Addit. Contam. Part A 2012, 29, 979–993. [Google Scholar] [CrossRef] [PubMed]
- Kuiper-Goodman, T.; Scott, P. Risk assessment of the mycotoxin ochratoxin A. Biomed. Environ. Sci. 1989, 2, 179–248. [Google Scholar] [PubMed]
- Al-Anati, L.; Petzinger, E. Immunotoxic activity of ochratoxin A. J. Vet. Pharmacol. Ther. 2006, 29, 79–90. [Google Scholar] [CrossRef] [PubMed]
- Belli, N.; Marin, S.; Sanchis, V.; Ramos, A. Review: Ochratoxin A (OTA) in wines, musts and grape juices: Occurrence, regulations and methods of analysis. Food Sci. Technol. Int. 2002, 8, 325–335. [Google Scholar] [CrossRef]
- Otteneder, H.; Majerus, P. Occurrence of ochratoxin A (OTA) in wines: Influence of the type of wine and its geographical origin. Food Addit. Contam. 2000, 17, 793–798. [Google Scholar] [CrossRef] [PubMed]
- Walker, R. Risk assessment of ochratoxin: Current views of the European Scientific Committee on Food, the JECFA and the Codex Committee on Food Additives and Contaminants. In Mycotoxins and Food Safety; Springer: Heidelberg, Germany, 2002; pp. 249–255. [Google Scholar]
- Santos, E.; Vargas, E. Immunoaffinity column clean-up and thin layer chromatography for determination of ochratoxin A in green coffee. Food Addit. Contam. 2002, 19, 447–458. [Google Scholar] [CrossRef] [PubMed]
- Tessini, C.; Mardones, C.; von Baer, D.; Vega, M.; Herlitz, E.; Saelzer, R.; Silva, J.; Torres, O. Alternatives for sample pre-treatment and HPLC determination of ochratoxin A in red wine using fluorescence detection. Anal. Chim. Acta 2010, 660, 119–126. [Google Scholar] [CrossRef] [PubMed]
- Olsson, J.; Börjesson, T.; Lundstedt, T.; Schnürer, J. Detection and quantification of ochratoxin A and deoxynivalenol in barley grains by GC-MS and electronic nose. Int. J. Food Microbiol. 2002, 72, 203–214. [Google Scholar] [CrossRef]
- Reinsch, M.; Töpfer, A.; Lehmann, A.; Nehls, I.; Panne, U. Determination of ochratoxin A in beer by LC-MS/MS ion trap detection. Food Chem. 2007, 100, 312–317. [Google Scholar] [CrossRef]
- Flajs, D.; Domijan, A.-M.; Ivić, D.; Cvjetković, B.; Peraica, M. ELISA and HPLC analysis of ochratoxin A in red wines of Croatia. Food Control 2009, 20, 590–592. [Google Scholar] [CrossRef]
- Chen, Z.; Tan, Y.; Zhang, C.; Yin, L.; Ma, H.; Ye, N.; Qiang, H.; Lin, Y. A colorimetric aptamer biosensor based on cationic polymer and gold nanoparticles for the ultrasensitive detection of thrombin. Biosens. Bioelectron. 2014, 56, 46–50. [Google Scholar] [CrossRef] [PubMed]
- O’Sullivan, C.K. Aptasensors—The future of biosensing? Anal. Bioanal. Chem. 2002, 372, 44–48. [Google Scholar] [CrossRef] [PubMed]
- Yang, C.; Wang, Y.; Marty, J.-L.; Yang, X. Aptamer-based colorimetric biosensing of Ochratoxin A using unmodified gold nanoparticles indicator. Biosens. Bioelectron. 2011, 26, 2724–2727. [Google Scholar] [CrossRef] [PubMed]
- Chen, J.; Fang, Z.; Liu, J.; Zeng, L. A simple and rapid biosensor for ochratoxin A based on a structure-switching signaling aptamer. Food Control 2012, 25, 555–560. [Google Scholar] [CrossRef]
- Wei, Y.; Zhang, J.; Wang, X.; Duan, Y.X. Amplified fluorescent aptasensor through catalytic recycling for highly sensitive detection of ochratoxin A. Biosens. Bioelectron. 2015, 65, 16–22. [Google Scholar] [CrossRef] [PubMed]
- Tombelli, S.; Minunni, M.; Mascini, M. Analytical applications of aptamers. Biosens. Bioelectron. 2005, 20, 2424–2434. [Google Scholar] [CrossRef] [PubMed]
- Jhaveri, S.; Rajendran, M.; Ellington, A.D. In vitro selection of signaling aptamers. Nat. Biotechnol. 2000, 18, 1293–1297. [Google Scholar] [PubMed]
- Han, S.R.; Yu, J.; Lee, S.-W. In vitro selection of RNA aptamers that selectively bind danofloxacin. Biochem. Biophys. Res. Commun. 2014, 448, 397–402. [Google Scholar] [CrossRef] [PubMed]
- Jeon, W.; Lee, S.; Ban, C. A colorimetric aptasensor for the diagnosis of malaria based on cationic polymers and gold nanoparticles. Anal. Biochem. 2013, 439, 11–16. [Google Scholar] [CrossRef] [PubMed]
- Song, S.; Wang, L.; Li, J.; Fan, C.; Zhao, J. Aptamer-based biosensors. TrAC Trends Anal. Chem. 2008, 27, 108–117. [Google Scholar] [CrossRef]
- Baggiani, C.; Giovannoli, C.; Anfossi, L. Man-Made Synthetic Receptors for Capture and Analysis of Ochratoxin A. Toxins 2015, 7, 4083–4098. [Google Scholar] [CrossRef] [PubMed]
- McKeague, M.; Velu, R.; Hill, K.; Bardoczy, V.; Meszaros, T.; DeRosa, M.C. Selection and characterization of a novel DNA aptamer for label-free fluorescence biosensing of ochratoxin A. Toxins 2014, 6, 2435–2452. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Rhouati, A.; Yang, C.; Hayat, A.; Marty, J.L. Aptamers: A promosing tool for ochratoxin A detection in food analysis. Toxins 2013, 5, 1988–2008. [Google Scholar] [CrossRef] [PubMed]
- Mishra, R.K.; Hayat, A.; Catanante, G.; Ocana, C.; Marty, J.L. A label free aptasensor for Ochratoxin A detection in cocoa beans: An application to chocolate industries. Anal. Chim. Acta 2015, 889, 106–112. [Google Scholar] [CrossRef] [PubMed]
- Wang, C.; Dong, X.; Liu, Q.; Wang, K. Label-free colorimetric aptasensor for sensitive detection of ochratoxin A utilizing hybridization chain reaction. Anal. Chim. Acta 2015, 860, 83–88. [Google Scholar] [CrossRef] [PubMed]
- Park, J.H.; Byun, J.Y.; Mun, H.; Shim, W.B.; Shin, Y.B.; Li, T.; Kim, M.G. A regeneratable, label-free, localized surface plasmon resonance (LSPR) aptasensor for the detection of ochratoxin A. Biosens. Bioelectron. 2014, 59, 321–327. [Google Scholar] [PubMed]
- Wu, Y.; Zhan, S.; Wang, F.; He, L.; Zhi, W.; Zhou, P. Cationic polymers and aptamers mediated aggregation of gold nanoparticles for the colorimetric detection of arsenic (III) in aqueous solution. Chem. Commun. 2012, 48, 4459–4461. [Google Scholar] [CrossRef] [PubMed]
- Cruz-Aguado, J.A.; Penner, G. Determination of ochratoxin a with a DNA aptamer. J. Agric. Food Chem. 2008, 56, 10456–10461. [Google Scholar] [CrossRef] [PubMed]
- Zhang, Y.; Peng, H.; Huang, W.; Zhou, Y.; Yan, D. Facile preparation and characterization of highly antimicrobial colloid Ag or Au nanoparticles. J. Colloid Interface Sci. 2008, 325, 371–376. [Google Scholar] [CrossRef] [PubMed]
- Chen, H.; Wang, Y.; Wang, Y.; Dong, S.; Wang, E. One-step preparation and characterization of PDDA-protected gold nanoparticles. Polymer 2006, 47, 763–766. [Google Scholar] [CrossRef]
- Marino-Repizo, L.; Kero, F.; Vandell, V.; Senior, A.; Isabel Sanz-Ferramola, M.; Cerutti, S.; Raba, J. A novel solid phase extraction-ultra high performance liquid chromatography-tandem mass spectrometry method for the quantification of ochratoxin A in red wines. Food Chem. 2015, 172, 663–668. [Google Scholar] [CrossRef] [PubMed]
- Zhang, X.; Ou, X.; Zhou, Z.; Ma, L. Ochratoxin A in Chinese dried jujube: Method development and survey. Food Addit. Contam. Part A 2015, 32, 512–517. [Google Scholar]
- Zezza, F.; Longobardi, F.; Pascale, M.; Eremin, S.A.; Visconti, A. Fluorescence polarization immunoassay for rapid screening of ochratoxin A in red wine. Anal. Bioanal. Chem. 2009, 395, 1317–1323. [Google Scholar] [CrossRef] [PubMed]
- Lippolis, V.; Pascale, M.; Valenzano, S.; Porricelli, A.C.R.; Suman, M.; Visconti, A. Fluorescence Polarization Immunoassay for Rapid, Accurate and Sensitive Determination of Ochratoxin A in Wheat. Food Anal. Methods 2014, 7, 298–307. [Google Scholar] [CrossRef]
- Cruz-Aguado, J.A.; Penner, G. Fluorescence Polarization Based Displacement Assay for the Determination of Small Molecules with Aptamers. Anal. Chem. 2008, 80, 8853–8855. [Google Scholar] [CrossRef] [PubMed]
- Yang, L.; Zhang, Y.; Li, R.; Lin, C.; Guo, L.; Qiu, B.; Lin, Z.; Chen, G. Electrochemiluminescence biosensor for ultrasensitive determination of ochratoxin A in corn samples based on aptamer and hyperbranched rolling circle amplification. Biosens. Bioelectron. 2015, 70, 268–274. [Google Scholar] [CrossRef] [PubMed]
- Mishra, R.K.; Hayat, A.; Catanante, G.; Istamboulie, G.; Marty, J.L. Sensitive quantitation of Ochratoxin A in cocoa beans using differential pulse voltammetry based aptasensor. Food Chem. 2016, 192, 799–804. [Google Scholar] [CrossRef] [PubMed]
- Yao, L.; Chen, Y.; Teng, J.; Zheng, W.; Wu, J.; Adeloju, S.B.; Pan, D.; Chen, W. Integrated platform with magnetic purification and rolling circular amplification for sensitive fluorescent detection of ochratoxin A. Biosens. Bioelectron. 2015, 74, 534–538. [Google Scholar] [CrossRef] [PubMed]
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Luan, Y.; Chen, J.; Li, C.; Xie, G.; Fu, H.; Ma, Z.; Lu, A. Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold Nanoparticles. Toxins 2015, 7, 5377-5385. https://doi.org/10.3390/toxins7124883
Luan Y, Chen J, Li C, Xie G, Fu H, Ma Z, Lu A. Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold Nanoparticles. Toxins. 2015; 7(12):5377-5385. https://doi.org/10.3390/toxins7124883
Chicago/Turabian StyleLuan, Yunxia, Jiayi Chen, Cheng Li, Gang Xie, Hailong Fu, Zhihong Ma, and Anxiang Lu. 2015. "Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold Nanoparticles" Toxins 7, no. 12: 5377-5385. https://doi.org/10.3390/toxins7124883