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Article

Phylloseptin-PBa—A Novel Broad-Spectrum Antimicrobial Peptide from the Skin Secretion of the Peruvian Purple-Sided Leaf Frog (Phyllomedusa Baltea) Which Exhibits Cancer Cell Cytotoxicity

1
Natural Drug Discovery Group, School of Pharmacy, Queen’s University, Belfast BT9 7BL, Northern Ireland, UK
2
College of Basic Medical Science, Zhejiang Chinese Medial University, Hangzhou 310053, China
3
Perubiotech Eirl, Santiago de Surco, Lima 33, Peru
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Stephen P. Mackessy
Toxins 2015, 7(12), 5182-5193; https://doi.org/10.3390/toxins7124878
Received: 14 October 2015 / Revised: 9 November 2015 / Accepted: 23 November 2015 / Published: 1 December 2015
(This article belongs to the Special Issue Venomics, Venom Proteomics and Venom Transcriptomics)
Antimicrobial peptides from amphibian skin secretion display remarkable broad-spectrum antimicrobial activity and are thus promising for the discovery of new antibiotics. In this study, we report a novel peptide belonging to the phylloseptin family of antimicrobial peptides, from the skin secretion of the purple-sided leaf frog, Phyllomedusa baltea, which was named Phylloseptin-PBa. Degenerate primers complementary to putative signal peptide sites of frog skin peptide precursor-encoding cDNAs were designed to interrogate a skin secretion-derived cDNA library from this frog. Subsequently, the peptide was isolated and identified using reverse phase HPLC and MS/MS fragmentation. The synthetic replicate was demonstrated to have activity against S. aureus, E. coli and C. albicans at concentrations of 8, 128 and 8 mg/L, respectively. In addition, it exhibited anti-proliferative activity against the human cancer cell lines, H460, PC3 and U251MG, but was less active against a normal human cell line (HMEC). Furthermore, a haemolysis assay was performed to assess mammalian cell cytotoxicity of Phylloseptin-PBa. This peptide contained a large proportion of α-helical domain, which may explain its antimicrobial and anticancer activities. View Full-Text
Keywords: amphibian; antimicrobial; anticancer; peptides; molecular cloning; mass spectrometry amphibian; antimicrobial; anticancer; peptides; molecular cloning; mass spectrometry
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MDPI and ACS Style

Wan, Y.; Ma, C.; Zhou, M.; Xi, X.; Li, L.; Wu, D.; Wang, L.; Lin, C.; Lopez, J.C.; Chen, T.; Shaw, C. Phylloseptin-PBa—A Novel Broad-Spectrum Antimicrobial Peptide from the Skin Secretion of the Peruvian Purple-Sided Leaf Frog (Phyllomedusa Baltea) Which Exhibits Cancer Cell Cytotoxicity. Toxins 2015, 7, 5182-5193. https://doi.org/10.3390/toxins7124878

AMA Style

Wan Y, Ma C, Zhou M, Xi X, Li L, Wu D, Wang L, Lin C, Lopez JC, Chen T, Shaw C. Phylloseptin-PBa—A Novel Broad-Spectrum Antimicrobial Peptide from the Skin Secretion of the Peruvian Purple-Sided Leaf Frog (Phyllomedusa Baltea) Which Exhibits Cancer Cell Cytotoxicity. Toxins. 2015; 7(12):5182-5193. https://doi.org/10.3390/toxins7124878

Chicago/Turabian Style

Wan, Yuantai, Chengbang Ma, Mei Zhou, Xinping Xi, Lei Li, Di Wu, Lei Wang, Chen Lin, Juan Chavez Lopez, Tianbao Chen, and Chris Shaw. 2015. "Phylloseptin-PBa—A Novel Broad-Spectrum Antimicrobial Peptide from the Skin Secretion of the Peruvian Purple-Sided Leaf Frog (Phyllomedusa Baltea) Which Exhibits Cancer Cell Cytotoxicity" Toxins 7, no. 12: 5182-5193. https://doi.org/10.3390/toxins7124878

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