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Toxins 2015, 7(1), 21-33;

Characterization of 27 Mycotoxin Binders and the Relation with in Vitro Zearalenone Adsorption at a Single Concentration

Department of Pharmacology, Toxicology and Biochemistry, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium
Department of Geology and Soil Science, Faculty of Science, Ghent University, Krijgslaan 281, S8, 9000 Ghent, Belgium
Department of Applied Biosciences, Faculty of Bioscience Engineering, Ghent University, Valentin Vaerwyckweg 1, 9000 Ghent, Belgium
Author to whom correspondence should be addressed.
Academic Editor: Annie Pfohl-Leszkowicz
Received: 11 September 2014 / Accepted: 23 December 2014 / Published: 5 January 2015
(This article belongs to the Special Issue Detoxification of Mycotoxins)
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The aim of this study was to characterize 27 feed additives marketed as mycotoxin binders and to screen them for their in vitro zearalenone (ZEN) adsorption. Firstly, 27 mycotoxin binders, commercially available in Belgium and The Netherlands, were selected and characterized. Characterization was comprised of X-ray diffraction (XRD) profiling of the mineral content and d-spacing, determination of the cation exchange capacity (CEC) and the exchangeable base cations, acidity, mineral fraction, relative humidity (RH) and swelling volume. Secondly, an in vitro screening experiment was performed to evaluate the adsorption of a single concentration of ZEN in a ZEN:binder ratio of 1:20,000. The free concentration of ZEN was measured after 4 h of incubation with each of the 27 mycotoxin binders at a pH of 2.5, 6.5 and 8.0. A significant correlation between the free concentration of ZEN and both the d-spacing and mineral fraction of the mycotoxin binders was seen at the three pH levels. A low free concentration of ZEN was demonstrated using binders containing mixed-layered smectites and binders containing humic acids. View Full-Text
Keywords: mycotoxin; binders; characterization; zearalenone; adsorption screening mycotoxin; binders; characterization; zearalenone; adsorption screening

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De Mil, T.; Devreese, M.; De Baere, S.; Van Ranst, E.; Eeckhout, M.; De Backer, P.; Croubels, S. Characterization of 27 Mycotoxin Binders and the Relation with in Vitro Zearalenone Adsorption at a Single Concentration. Toxins 2015, 7, 21-33.

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