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Open AccessArticle

Aspergillus Oxylipin Signaling and Quorum Sensing Pathways Depend on G Protein-Coupled Receptors

1
Department of Bacteriology and Department of Medical Microbiology and Immunology, 1550 Linden Drive, Madison, WI 53706, USA
2
Department of Biology, Western Washington University, 516 High Street, Bellingham, WA 98225, USA
*
Author to whom correspondence should be addressed.
Toxins 2012, 4(9), 695-717; https://doi.org/10.3390/toxins4090695
Received: 3 August 2012 / Revised: 31 August 2012 / Accepted: 31 August 2012 / Published: 18 September 2012
(This article belongs to the Special Issue Mycotoxins in Food and Feed)
Oxylipins regulate Aspergillus development and mycotoxin production and are also involved in Aspergillus quorum sensing mechanisms. Despite extensive knowledge of how these oxylipins are synthesized and what processes they regulate, nothing is known about how these signals are detected and transmitted by the fungus. G protein-coupled receptors (GPCR) have been speculated to be involved as they are known oxylipin receptors in mammals, and many putative GPCRs have been identified in the Aspergilli. Here, we present evidence that oxylipins stimulate a burst in cAMP in A. nidulans, and that loss of an A. nidulans GPCR, gprD, prevents this cAMP accumulation. A. flavus undergoes an oxylipin-mediated developmental shift when grown at different densities, and this regulates spore, sclerotial and aflatoxin production. A. flavus encodes two putative GprD homologs, GprC and GprD, and we demonstrate here that they are required to transition to a high-density development state, as well as to respond to spent medium of a high-density culture. The finding of GPCRs that regulate production of survival structures (sclerotia), inoculum (spores) and aflatoxin holds promise for future development of anti-fungal therapeutics. View Full-Text
Keywords: aflatoxin; Aspergillus; oxylipin; G protein-coupled receptor (GPCR); quorum sensing; sclerotia aflatoxin; Aspergillus; oxylipin; G protein-coupled receptor (GPCR); quorum sensing; sclerotia
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Affeldt, K.J.; Brodhagen, M.; Keller, N.P. Aspergillus Oxylipin Signaling and Quorum Sensing Pathways Depend on G Protein-Coupled Receptors. Toxins 2012, 4, 695-717.

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