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Article

Kinetics, Thermodynamics and Mechanism of Enzymatic Degradation of Zearalenone in Degummed Corn Oil

by
Chenwei Zhao
,
Pengkai Xie
,
Jun Jin
,
Qingzhe Jin
* and
Xingguo Wang
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
*
Author to whom correspondence should be addressed.
Toxins 2023, 15(1), 19; https://doi.org/10.3390/toxins15010019
Submission received: 11 November 2022 / Revised: 19 December 2022 / Accepted: 21 December 2022 / Published: 28 December 2022
(This article belongs to the Section Mycotoxins)
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Abstract

The kinetics and thermodynamics of the enzymatic degradation of zearalenone (ZEN) in degummed corn oil were investigated by analyzing the impacts of temperature, pH, ZEN hydrolase dosage and ZEN concentration on the initial reaction rate. The kinetic study found that the maximum reaction rate was 0.97 μmol × kg−1 min−1, the Michaelis constant (Km) was 11,476 μmol × kg−1 and the Michaelis equation was V = 0.97[S]/(11,476 + [S]). The thermodynamic study showed that the activation energy (Ea) was 70.37 kJ·mol−1, the activation enthalpy change of the reaction (ΔH) > 0, the free energy of activation (ΔG) > 0 and the activation entropy change (ΔS) < 0, indicating the reaction could not be spontaneous. The reaction mechanism of ZEN was studied by a hybrid quadrupole orbitrap mass spectrometer. It was found that ZEN first generated the intermediate G/L/D/W-ZEN+H2O, followed by generating the intermediate W-ZEN-H2O under the action of a degrading enzyme. Then, the lactone bond was opened to produce C18H24O6, and finally the decarboxylation product C17H24O4 formed automatically.
Keywords: zearalenone; hydrolase; degummed corn oil; kinetics; thermodynamic; mechanism zearalenone; hydrolase; degummed corn oil; kinetics; thermodynamic; mechanism

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MDPI and ACS Style

Zhao, C.; Xie, P.; Jin, J.; Jin, Q.; Wang, X. Kinetics, Thermodynamics and Mechanism of Enzymatic Degradation of Zearalenone in Degummed Corn Oil. Toxins 2023, 15, 19. https://doi.org/10.3390/toxins15010019

AMA Style

Zhao C, Xie P, Jin J, Jin Q, Wang X. Kinetics, Thermodynamics and Mechanism of Enzymatic Degradation of Zearalenone in Degummed Corn Oil. Toxins. 2023; 15(1):19. https://doi.org/10.3390/toxins15010019

Chicago/Turabian Style

Zhao, Chenwei, Pengkai Xie, Jun Jin, Qingzhe Jin, and Xingguo Wang. 2023. "Kinetics, Thermodynamics and Mechanism of Enzymatic Degradation of Zearalenone in Degummed Corn Oil" Toxins 15, no. 1: 19. https://doi.org/10.3390/toxins15010019

APA Style

Zhao, C., Xie, P., Jin, J., Jin, Q., & Wang, X. (2023). Kinetics, Thermodynamics and Mechanism of Enzymatic Degradation of Zearalenone in Degummed Corn Oil. Toxins, 15(1), 19. https://doi.org/10.3390/toxins15010019

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