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Article

The Crystal Structure of Bacillus cereus HblL1

1
School of Biosciences, Cardiff University, Park Place, Cardiff CF10 3AX, UK
2
The Henry Wellcome Building for Biocatalysis, Exeter University, Stocker Road, Exeter EX4 4QD, UK
3
Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq
4
Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
5
Scientific Review Program, Division of Extramural Activities, NIAID, NIH, Rockville, MD 20892, USA
6
School of Medicine, Cardiff University, Heath Campus, Cardiff CF14 4XN, UK
*
Author to whom correspondence should be addressed.
Toxins 2021, 13(4), 253; https://doi.org/10.3390/toxins13040253
Received: 2 March 2021 / Revised: 26 March 2021 / Accepted: 29 March 2021 / Published: 31 March 2021
(This article belongs to the Special Issue Bacillus cereus Toxins)
The Hbl toxin is a three-component haemolytic complex produced by Bacillus cereus sensu lato strains and implicated as a cause of diarrhoea in B. cereus food poisoning. While the structure of the HblB component of this toxin is known, the structures of the other components are unresolved. Here, we describe the expression of the recombinant HblL1 component and the elucidation of its structure to 1.36 Å. Like HblB, it is a member of the alpha-helical pore-forming toxin family. In comparison to other members of this group, it has an extended hydrophobic beta tongue region that may be involved in pore formation. Molecular docking was used to predict possible interactions between HblL1 and HblB, and suggests a head to tail dimer might form, burying the HblL1 beta tongue region. View Full-Text
Keywords: tripartite toxin; hemolytic toxin; haemolytic toxin; Bacillus cereus tripartite toxin; hemolytic toxin; haemolytic toxin; Bacillus cereus
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MDPI and ACS Style

Worthy, H.L.; Williamson, L.J.; Auhim, H.S.; Leppla, S.H.; Sastalla, I.; Jones, D.D.; Rizkallah, P.J.; Berry, C. The Crystal Structure of Bacillus cereus HblL1. Toxins 2021, 13, 253. https://doi.org/10.3390/toxins13040253

AMA Style

Worthy HL, Williamson LJ, Auhim HS, Leppla SH, Sastalla I, Jones DD, Rizkallah PJ, Berry C. The Crystal Structure of Bacillus cereus HblL1. Toxins. 2021; 13(4):253. https://doi.org/10.3390/toxins13040253

Chicago/Turabian Style

Worthy, Harley L.; Williamson, Lainey J.; Auhim, Husam S.; Leppla, Stephen H.; Sastalla, Inka; Jones, D. D.; Rizkallah, Pierre J.; Berry, Colin. 2021. "The Crystal Structure of Bacillus cereus HblL1" Toxins 13, no. 4: 253. https://doi.org/10.3390/toxins13040253

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