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An Optical Planar Waveguide-Based Immunosensors for Determination of Fusarium Mycotoxin Zearalenone
Open AccessArticle

Development of an Immunofluorescence Assay Module for Determination of the Mycotoxin Zearalenone in Water

1
Agro-Environmental Research Centre, Institute of Environmental Sciences, Hungarian University of Agriculture and Life Sciences, Herman O. út 15, H-1022 Budapest, Hungary
2
Department of Atomic Physics, Budapest University of Technology and Economics, Műegyetem rkp. 3, H-1111 Budapest, Hungary
3
Optimal Optik Ltd., Dayka Gábor u. 6/B, H-1118 Budapest, Hungary
4
Food Science Research Centre, Institute of Food Sciences, Hungarian University of Agriculture and Life Sciences, Herman O. út 15, H-1022 Budapest, Hungary
5
Materials and Engineering Research Institute, Sheffield Hallam University, Howard Street, Sheffield S1 1WB, UK
*
Author to whom correspondence should be addressed.
Toxins 2021, 13(3), 182; https://doi.org/10.3390/toxins13030182
Received: 10 February 2021 / Revised: 23 February 2021 / Accepted: 24 February 2021 / Published: 2 March 2021
(This article belongs to the Special Issue Rapid Detection of Mycotoxin Contamination)
Project Aquafluosense is designed to develop prototypes for a fluorescence-based instrumentation setup for in situ measurements of several characteristic parameters of water quality. In the scope of the project an enzyme-linked fluorescent immunoassay (ELFIA) method has been developed for the detection of several environmental xenobiotics, including mycotoxin zearalenone (ZON). ZON, produced by several plant pathogenic Fusarium species, has recently been identified as an emerging pollutant in surface water, presenting a hazard to aquatic ecosystems. Due to its physico-chemical properties, detection of ZON at low concentrations in surface water is a challenging task. The 96-well microplate-based fluorescence instrument is capable of detecting ZON in the concentration range of 0.09–400 ng/mL. The sensitivity and accuracy of the analytical method has been demonstrated by a comparative assessment with detection by high-performance liquid chromatography and by total internal reflection ellipsometry. The limit of detection of the method, 0.09 ng/mL, falls in the low range compared to the other reported immunoassays, but the main advantage of this ELFIA method is its efficacy in combined in situ applications for determination of various important water quality parameters detectable by induced fluorimerty—e.g., total organic carbon content, algal density or the level of other organic micropollutants detectable by immunofluorimetry. In addition, the immunofluorescence module can readily be expanded to other target analytes if proper antibodies are available for detection. View Full-Text
Keywords: zearalenone; mycotoxin; competitive immunoassay; fluorescence detection; high-performance liquid chromatography; total internal reflection ellipsometry zearalenone; mycotoxin; competitive immunoassay; fluorescence detection; high-performance liquid chromatography; total internal reflection ellipsometry
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MDPI and ACS Style

Gémes, B.; Takács, E.; Gádoros, P.; Barócsi, A.; Kocsányi, L.; Lenk, S.; Csákányi, A.; Kautny, S.; Domján, L.; Szarvas, G.; Adányi, N.; Nabok, A.; Mörtl, M.; Székács, A. Development of an Immunofluorescence Assay Module for Determination of the Mycotoxin Zearalenone in Water. Toxins 2021, 13, 182. https://doi.org/10.3390/toxins13030182

AMA Style

Gémes B, Takács E, Gádoros P, Barócsi A, Kocsányi L, Lenk S, Csákányi A, Kautny S, Domján L, Szarvas G, Adányi N, Nabok A, Mörtl M, Székács A. Development of an Immunofluorescence Assay Module for Determination of the Mycotoxin Zearalenone in Water. Toxins. 2021; 13(3):182. https://doi.org/10.3390/toxins13030182

Chicago/Turabian Style

Gémes, Borbála; Takács, Eszter; Gádoros, Patrik; Barócsi, Attila; Kocsányi, László; Lenk, Sándor; Csákányi, Attila; Kautny, Szabolcs; Domján, László; Szarvas, Gábor; Adányi, Nóra; Nabok, Alexei; Mörtl, Mária; Székács, András. 2021. "Development of an Immunofluorescence Assay Module for Determination of the Mycotoxin Zearalenone in Water" Toxins 13, no. 3: 182. https://doi.org/10.3390/toxins13030182

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