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Open AccessArticle

Crystal Structure of Exotoxin A from Aeromonas Pathogenic Species

Department of Pharmacy and Pharmacology, Centre for Therapeutic Innovation, University of Bath, Bath BA2 7AY, UK
Toxins 2020, 12(6), 397; https://doi.org/10.3390/toxins12060397
Received: 26 May 2020 / Revised: 11 June 2020 / Accepted: 12 June 2020 / Published: 15 June 2020
Aeromonas exotoxin A (AE) is a bacterial virulence factor recently discovered in a clinical case of necrotising fasciitis caused by the flesh-eating Aeromonas hydrophila. Here, database mining shows that AE is present in the genome of several emerging Aeromonas pathogenic species. The X-ray crystal structure of AE was solved at 2.3 Å and presents all the hallmarks common to diphthamide-specific mono-ADP-ribosylating toxins, suggesting AE is a fourth member of this family alongside the diphtheria toxin, Pseudomonas exotoxin A and cholix. Structural homology indicates AE may use a similar mechanism of cytotoxicity that targets eukaryotic elongation factor 2 and thus inhibition of protein synthesis. The structure of AE also highlights unique features including a metal binding site, and a negatively charged cleft that could play a role in interdomain interactions and may affect toxicity. This study raises new opportunities to engineer alternative toxin-based molecules with pharmaceutical potential. View Full-Text
Keywords: ADP-ribosyltransferases; exotoxin; bacterial toxin; X-ray crystal structure; Aeromonas ADP-ribosyltransferases; exotoxin; bacterial toxin; X-ray crystal structure; Aeromonas
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MDPI and ACS Style

Masuyer, G. Crystal Structure of Exotoxin A from Aeromonas Pathogenic Species. Toxins 2020, 12, 397.

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