Next Article in Journal
The Nuclear Remodeling Induced by Helicobacter Cytolethal Distending Toxin Involves MAFB Oncoprotein
Previous Article in Journal
Mycotoxin Exposure and Related Diseases
Open AccessArticle

An Acetylcholinesterase Inhibition-Based Biosensor for Aflatoxin B1 Detection Using Sodium Alginate as an Immobilization Matrix

1
Department of Chemistry, College of Science Al-Zulfi, Majmaah University, Al-Majmaah 11952, Saudi Arabia
2
Laboratory of Interfaces and Advanced Materials, Faculty of Sciences, Monastir University, Monastir 5000, Tunisia
3
Institute of Analytical Sciences, UMR CNRS-UCBL-ENS 5280, 5 Rue la Doua, 69100 Villeurbanne, CEDEX, France
4
Faculty of Sciences of Tunis, Tunis El Manar University, El Manar, Tunis 1068, Tunis
5
Department of Basic Sciences, Adham University College, Umm Al-Qura University, Adham 21971, Saudi Arabia
6
Department of Biology, Faculty of Applied Sciences, Umm Al-Qura University, Makkah 21955, Saudi Arabia
7
Research Laboratories Center, Faculty of Applied Science, Umm Al-Qura University, Mecca 21955, Saudi Arabia
8
Agricultural Genetic Engineering Research Institute (AGERI), ARC, Giza 12915, Egypt
9
Department of Biology, Aljumum University College, Umm Al-Qura University, Makkah Aljumum 21955, Saudi Arabia
10
Department of Biology, Faculty of Sciences and Arts, King Khalid University, Dhahran Aljanoub 61421, Saudi Arabia
11
Department of Chemistry, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11432, Saudi Arabia
*
Author to whom correspondence should be addressed.
Toxins 2020, 12(3), 173; https://doi.org/10.3390/toxins12030173
Received: 12 February 2020 / Revised: 2 March 2020 / Accepted: 5 March 2020 / Published: 11 March 2020
In this study, we investigated a novel aflatoxin biosensor based on acetylcholinesterase (AChE) inhibition by aflatoxin B1 (AFB1) and developed electrochemical biosensors based on a sodium alginate biopolymer as a new matrix for acetylcholinesterase immobilization. Electrochemical impedance spectroscopy was performed as a convenient transduction method to evaluate the AChE activity through the oxidation of the metabolic product, thiocholine. Satisfactory analytical performances in terms of high sensitivity, good repeatability, and long-term storage stability were obtained with a linear dynamic range from 0.1 to 100 ng/mL and a low detection limit of 0.1 ng/mL, which is below the recommended level of AFB1 (2 µg/L). The suitability of the proposed method was evaluated using the samples of rice supplemented with AFB1 (0.5 ng/mL). The selectivity of the AChE-biosensor for aflatoxins relative to other sets of toxic substances (OTA, AFM 1) was also investigated. View Full-Text
Keywords: biosensor; acetylcholinesterase; aflatoxin B 1; sodium alginate; biopolymer biosensor; acetylcholinesterase; aflatoxin B 1; sodium alginate; biopolymer
Show Figures

Graphical abstract

MDPI and ACS Style

Chrouda, A.; Zinoubi, K.; Soltane, R.; Alzahrani, N.; Osman, G.; Al-Ghamdi, Y.O.; Qari, S.; Al Mahri, A.; Algethami, F.K.; Majdoub, H.; Jaffrezic Renault, N. An Acetylcholinesterase Inhibition-Based Biosensor for Aflatoxin B1 Detection Using Sodium Alginate as an Immobilization Matrix. Toxins 2020, 12, 173.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop