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Open AccessArticle

In Vitro Immunological Cross-Reactivity of Thai Polyvalent and Monovalent Antivenoms with Asian Viper Venoms

1
Department of Pharmacology, Phramongkutklao College of Medicine, Bangkok 10400, Thailand
2
Kulliyyah of Pharmacy, International Islamic University Malaysia, Kuantan Campus, Bandar Indera Mahkota, Kuantan 25200, Pahang Darul Makmur, Malaysia
3
Centre for Snakebite Research & Interventions, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, Merseyside L3 5QA, UK
4
Jeffrey Cheah School of Medicine and Health Sciences, Monash University Sunway Campus, Bandar Sunway 46150, Malaysia
*
Authors to whom correspondence should be addressed.
Toxins 2020, 12(12), 766; https://doi.org/10.3390/toxins12120766
Received: 30 September 2020 / Revised: 16 November 2020 / Accepted: 28 November 2020 / Published: 3 December 2020
(This article belongs to the Special Issue Novel Strategies for the Diagnosis and Treatment of Snakebites)
The intravenous administration of polyclonal antibodies known as antivenom is the only effective treatment for snakebite envenomed victims, but because of inter-specific variation in the toxic components of snake venoms, these therapies have variable efficacies against different snake species and/or different populations of the same species. In this study, we sought to characterize the in vitro venom binding capability and in vitro cross-neutralizing activity of antivenom, specifically the Hemato Polyvalent antivenom (HPAV; The Queen Saovabha Memorial Institute (QSMI) of the Thai Red Cross Society, Thailand) and three monovalent antivenoms (QSMI) specific to Daboia siamensis, Calloselasma rhodostoma, and Trimeresurus albolabris venoms, against a variety of South Asian and Southeast Asian viper venoms (Calloselasma rhodostoma, Daboia russelii, Hypnale hypnale, Trimeresurus albolabris, Trimeresurus purpureomaculatus, Trimeresurus hageni, and Trimeresurus fucatus). Using ELISA and immunoblotting approaches, we find that the majority of protein components in the viper venoms were recognized and bound by the HPAV polyvalent antivenom, while the monospecific antivenom made against T.albolabris extensively recognized toxins present in the venom of related species, T. purpureomaculatus, T. hageni, and T. fucatus. In vitro coagulation assays using bovine plasma revealed similar findings, with HPAV antivenom significantly inhibiting the coagulopathic activities of all tested viper venoms and T. albolabris antivenom inhibiting the venoms from Malaysian arboreal pit vipers. We also show that the monovalent C. rhodostoma antivenom exhibits highly comparable levels of immunological binding and in vitro venom neutralization to venom from both Thailand and Malaysia, despite previous reports of considerable intraspecific venom variation. Our findings suggest that Thai antivenoms from QSMI may by useful therapeutics for managing snake envenomings caused by a number of Southeast Asian viper species and populations for which no specific antivenom currently exists and thus should be explored further to assess their clinical utility in treating snakebite victims. View Full-Text
Keywords: snakebite; venom; vipers; antivenom; cross-neutralization; coagulation; ELISA snakebite; venom; vipers; antivenom; cross-neutralization; coagulation; ELISA
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MDPI and ACS Style

Chaisakul, J.; Rusmili, M.R.A.; Alsolaiss, J.; Albulescu, L.-O.; Harrison, R.A.; Othman, I.; Casewell, N.R. In Vitro Immunological Cross-Reactivity of Thai Polyvalent and Monovalent Antivenoms with Asian Viper Venoms. Toxins 2020, 12, 766. https://doi.org/10.3390/toxins12120766

AMA Style

Chaisakul J, Rusmili MRA, Alsolaiss J, Albulescu L-O, Harrison RA, Othman I, Casewell NR. In Vitro Immunological Cross-Reactivity of Thai Polyvalent and Monovalent Antivenoms with Asian Viper Venoms. Toxins. 2020; 12(12):766. https://doi.org/10.3390/toxins12120766

Chicago/Turabian Style

Chaisakul, Janeyuth; Rusmili, Muhamad R.A.; Alsolaiss, Jaffer; Albulescu, Laura-Oana; Harrison, Robert A.; Othman, Iekhsan; Casewell, Nicholas R. 2020. "In Vitro Immunological Cross-Reactivity of Thai Polyvalent and Monovalent Antivenoms with Asian Viper Venoms" Toxins 12, no. 12: 766. https://doi.org/10.3390/toxins12120766

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