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Open AccessArticle

Occurrence and Characterization of Fungi and Mycotoxins in Contaminated Medicinal Herbs

State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China
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Toxins 2020, 12(1), 30; https://doi.org/10.3390/toxins12010030 (registering DOI)
Received: 1 November 2019 / Revised: 24 December 2019 / Accepted: 26 December 2019 / Published: 3 January 2020
(This article belongs to the Section Mycotoxins)
Traditional medicinal herbs are widely used and may be contaminated with mycotoxigenic fungi during cultivation, harvesting, and storage, causing spoilage and mycotoxin production. We evaluated the predominant mycoflora and extent of mycotoxin contaminations in 48 contaminated samples of 13 different medicinal herbs. In total, 70.8% of herbs were slightly contaminated with aflatoxins (<5 μg kg−1). Codonopsis radix samples contained ochratoxin A (OTA) (360–515 μg kg−1), and Scutellariae radix samples contained OTA (49–231 μg kg−1) and citrinin (15–53 μg kg−1). Forty samples (83.3%) contained fungal contamination. Sixty-nine strains were characterized via morphological and molecular identification. The predominant mycoflora comprised four genera, Aspergillus spp. (26.1%), Penicillium spp. (24.6%), Rhizopus spp. (14.5%), and Trichoderma spp. (11.6%). Aflatoxins, OTA, and citrinin were detected in 37 cultures by high-performance liquid chromatography-tandem mass spectrometry. Approximately 21.6% of Aspergillus and Penicillium isolates produced mycotoxins. One Penicillium polonicum strain isolated from Scutellariae radix synthesized citrinin. Multiplex PCR analysis showed that three Aspergillus flavus strains harbored aflatoxin biosynthesis genes. One Aspergillus flavus strain isolated from Amomi fructus produced AFB1 and AFB2. To the best of our knowledge, the citrinin production by Aspergillus chevalieri and Penicillium sacculum was first reported in this study, which poses a potential risk of mycotoxin contamination in medicinal herbs. View Full-Text
Keywords: mycoflora; medicinal herbs; mycotoxin; contamination; aflatoxins; citrinin; mycotoxigenic fungi; multiplex PCR; HPLC-MS/MS mycoflora; medicinal herbs; mycotoxin; contamination; aflatoxins; citrinin; mycotoxigenic fungi; multiplex PCR; HPLC-MS/MS
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Chen, L.; Guo, W.; Zheng, Y.; Zhou, J.; Liu, T.; Chen, W.; Liang, D.; Zhao, M.; Zhu, Y.; Wu, Q.; Zhang, J. Occurrence and Characterization of Fungi and Mycotoxins in Contaminated Medicinal Herbs. Toxins 2020, 12, 30.

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