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Open AccessCommunication

Heterologous Expression and Characterization of A Novel Ochratoxin A Degrading Enzyme, N-acyl-L-amino Acid Amidohydrolase, from Alcaligenes faecalis

1
Gansu Key Laboratory of Viticulture and Enology, College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China
2
Academy of State, Administration of Grain, Beijing 100032, China
3
Beijing Key Laboratory of Nutrition and Health and Food Safety, COFCO Nutrition and Health Institute, Beijing 102209, China
*
Authors to whom correspondence should be addressed.
Toxins 2019, 11(9), 518; https://doi.org/10.3390/toxins11090518
Received: 28 July 2019 / Revised: 1 September 2019 / Accepted: 5 September 2019 / Published: 6 September 2019
(This article belongs to the Section Mycotoxins)
Ochratoxin A (OTA) is a well-known, natural contaminant in foods and feeds because of its toxic effects, such as nephrotoxicity in various animals. Recent studies have revealed that Alcaligenes faecalis could generate enzymes to efficiently degrade OTA to ochratoxin α (OTα) in vitro. In an effort to obtain the OTA degrading mechanism, we purified and identified a novel degrading enzyme, N-acyl-L-amino acid amidohydrolase (AfOTase), from A. faecalis DSM 16503 via mass spectrometry. The same gene of the enzyme was also encountered in other A. faecalis strains. AfOTase belongs to peptidase family M20 and contains metal ions at the active site. In this study, recombination AfOTase was expressed and characterized in Escherichia coli. The molecular mass of recombinant rAfOTase was approximately 47.0 kDa, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The enzyme exhibited a wide temperature range (30–70 °C) and pH adaptation (4.5–9.0) and the optimal temperature and pH were 50 °C and 6.5, respectively. View Full-Text
Keywords: ochratoxin A; degradation; amidohydrolase; Alcaligenes faecalis; mycotoxin ochratoxin A; degradation; amidohydrolase; Alcaligenes faecalis; mycotoxin
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Zhang, H.; Zhang, Y.; Yin, T.; Wang, J.; Zhang, X. Heterologous Expression and Characterization of A Novel Ochratoxin A Degrading Enzyme, N-acyl-L-amino Acid Amidohydrolase, from Alcaligenes faecalis. Toxins 2019, 11, 518.

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