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Open AccessArticle

Serrulin: A Glycine-Rich Bioactive Peptide from the Hemolymph of the Yellow Tityus serrulatus Scorpion

1
Special Laboratory for Applied Toxinology (LETA), Butantan Institute, São Paulo CEP 05503-900, SP, Brazil
2
Institute of Biomedical Sciences, University of São Paulo, São Paulo CEP 05508-900, SP, Brazil
*
Authors to whom correspondence should be addressed.
Toxins 2019, 11(9), 517; https://doi.org/10.3390/toxins11090517
Received: 26 June 2019 / Revised: 31 July 2019 / Accepted: 5 August 2019 / Published: 6 September 2019
Antimicrobial peptides (AMPs) are small molecules, which have a potential use as antibiotic or pharmacological tools. In chelicerate organisms, such as scorpions, these molecules constitute an alternative defense system against microorganisms. The aim of this work was to identify AMPs in the hemolymph of the Tityus serrulatus scorpion. Fractions of plasma and hemocytes were subjected to high-performance liquid chromatography (HPLC) and then analyzed to determine their activity in inhibiting microbial growth. One of the fractions from the hemocytes presents antimicrobial activity against microorganisms, such as Gram-negative and Gram-positive bacteria, fungi, and yeast. These fractions were analyzed by mass spectrometry, and a fragment of 3564 Da. was identified. The peptide was called serrulin, because it is derived from the species T. serrulatus. A comparison of the amino acid sequence of serrulin with databases shows that it has a similarity to the glycine-rich peptides described in Cupienius salai and Acanthoscurria gomesiana (spiders). Furthermore, serrulin has no hemolytic activity against human erythrocytes. While the presence of AMPs in T. serrulatus venom has been described in other works, this is the first work to characterize the presence of these molecules in the hemolymph (hemocytes) of this species and show its potential use as an alternative to conventional antibiotics against different species of microorganisms. View Full-Text
Keywords: antimicrobial peptide; glycine-rich peptide; innate immune system; scorpions; Tityus serrulatus antimicrobial peptide; glycine-rich peptide; innate immune system; scorpions; Tityus serrulatus
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MDPI and ACS Style

de Jesus Oliveira, T.; Oliveira, U.C.; da Silva Junior, P.I. Serrulin: A Glycine-Rich Bioactive Peptide from the Hemolymph of the Yellow Tityus serrulatus Scorpion. Toxins 2019, 11, 517.

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