Next Article in Journal
Efficacy of Azoxystrobin on Mycotoxins and Related Fungi in Italian Paddy Rice
Next Article in Special Issue
Aflatoxin in Chili Peppers in Nigeria: Extent of Contamination and Control Using Atoxigenic Aspergillus flavus Genotypes as Biocontrol Agents
Previous Article in Journal
Occurrence of Deoxynivalenol and Ochratoxin A in Beers and Wines Commercialized in Paraguay
Previous Article in Special Issue
Multiple CH/π Interactions Maintain the Binding of Aflatoxin B1 in the Active Cavity of Human Cytochrome P450 1A2
Open AccessArticle

Separation and Purification of Aflatoxins by Centrifugal Partition Chromatography

1
Departement of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary
2
Doctoral School in Biology, Faculty of Science and Informatics, University of Szeged, H-6720 Szeged, Hungary
3
Faculty of Technology, University of Novi Sad, Bulevar cara Lazara 1, 21000 Novi Sad, Serbia
*
Author to whom correspondence should be addressed.
Toxins 2019, 11(6), 309; https://doi.org/10.3390/toxins11060309
Received: 21 May 2019 / Revised: 25 May 2019 / Accepted: 27 May 2019 / Published: 30 May 2019
(This article belongs to the Collection Aflatoxins)
Aflatoxins are mycotoxins that are produced by several species of filamentous fungi. In the European Union, the concentration limits for this group of mycotoxins in food and feed products are very low (on the order of parts per billion). Thus, relatively high amounts of these substances in their pure forms are required as reference standards. Chromatographic techniques based on solid stationary phases are generally used to purify these molecules; however, liquid–liquid chromatographic separations may be a promising alternative. Therefore, this study proposes a liquid–liquid chromatographic method for the separation of four aflatoxins and impurities. To optimise the method, numerous biphasic solvent systems (chloroform-, acetone- and acetic acid-based systems) were tested and evaluated in terms of their effectiveness at partitioning aflatoxins; the toluene/acetic acid/water (30:24:50, v/v/v/%) system was found to be the most efficient for application in centrifugal partition chromatographic instrument. Using liquid–liquid instrumental separation, the four aflatoxins, namely B1 (400 mg), B2 (34 mg), G1 (817 mg) and G2 (100 mg), were successfully isolated with 96.3%–98.2% purity from 4.5 L of Aspergillus parasiticus fermented material in a 250 mL centrifugal partition chromatography column. The identities and purities of the purified components were confirmed, and the performance parameters of each separation step and the whole procedure was determined. The developed method could be effectively used to purify aflatoxins for analytical applications. View Full-Text
Keywords: aflatoxin purification; centrifugal partition chromatography; ternary system; separation aflatoxin purification; centrifugal partition chromatography; ternary system; separation
Show Figures

Figure 1

MDPI and ACS Style

Endre, G.; Hegedüs, Z.; Turbat, A.; Škrbić, B.; Vágvölgyi, C.; Szekeres, A. Separation and Purification of Aflatoxins by Centrifugal Partition Chromatography. Toxins 2019, 11, 309.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop