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Open AccessArticle

Effects of Different Carbon Sources on Fumonisin Production and FUM Gene Expression by Fusarium proliferatum

1
Key Laboratory of Plant Resource Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, Key Laboratory of Post-harvest Handling of Fruits, Ministry of Agriculture, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
2
School of Life Sciences, University of Chinese Academy of Sciences, Beijing 100039, China
3
Zhongshan Entry-Exit Inspection and Quarantine Bureau, Zhongshan 528403, China
*
Author to whom correspondence should be addressed.
Toxins 2019, 11(5), 289; https://doi.org/10.3390/toxins11050289
Received: 14 April 2019 / Revised: 9 May 2019 / Accepted: 14 May 2019 / Published: 22 May 2019
Fusarium proliferatum can infect many crops and then produce fumonisins that are very harmful to humans and animals. Previous study indicates that carbon sources play important roles in regulating the fumonisin biosynthesis. Unfortunately, there is limited information on the effects of carbon starvation in comparison with the carbon sources present in the host of fumonisin production in F. proliferatum. Our results indicated that F. proliferatum cultivated in the Czapek’s broth (CB) medium in the absence of sucrose could greatly induce production of fumonisin, while an additional supplementation of sucrose to the culture medium significantly reduced the fumonisin production. Furthermore, cellulose and hemicellulose, and polysaccharide extracted from banana peel, which replaced sucrose as the carbon source, can reduce the production of fumonisin by F. proliferatum. Further work showed that these genes related to the synthesis of fumonisin, such as FUM1 and FUM8, were significantly up-regulated in the culture medium in the absence of sucrose. Consistent with fumonisin production, the expressions of FUM gene cluster and ZFR1 gene decreased after the addition of sucrose. Moreover, these genes were also significantly down-regulated in the presence of cellulose, hemicellulose or polysaccharide extracted from peel. Altogether, our results suggested that fumonisin production was regulated in F. proliferatum in response to different carbon source conditions, and this regulation might be mainly via the transcriptional level. Future work on these expressions of the fumonisin biosynthesis-related genes is needed to further clarify the response under different carbon conditions during the infection of F. proliferatum on banana fruit hosts. The findings in this study will provide a new clue regarding the biological effect of the fumonisin production in response to environmental stress. View Full-Text
Keywords: Fusarium proliferatum; fumonisin biosynthesis; carbon source; environmental stress; gene expression Fusarium proliferatum; fumonisin biosynthesis; carbon source; environmental stress; gene expression
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Wu, Y.; Li, T.; Gong, L.; Wang, Y.; Jiang, Y. Effects of Different Carbon Sources on Fumonisin Production and FUM Gene Expression by Fusarium proliferatum. Toxins 2019, 11, 289.

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