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Fusaoctaxin A, an Example of a Two-Step Mechanism for Non-Ribosomal Peptide Assembly and Maturation in Fungi

1
Department of Chemistry and Bioscience, Aalborg University, 9220 Aalborg, Denmark
2
Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Konrad-Lorenz-Straße 24, Tulln an der Donau 3430, Austria
3
Division of Biotechnology and Plant Health, Norwegian Institute of Bioeconomy Research, 1433 Ås, Norway
4
Department of Chemistry and Bioscience, Aalborg University, Niels Bohrs Vej 8, 6700 Esbjerg, Denmark
*
Author to whom correspondence should be addressed.
Toxins 2019, 11(5), 277; https://doi.org/10.3390/toxins11050277
Received: 5 March 2019 / Revised: 13 May 2019 / Accepted: 14 May 2019 / Published: 16 May 2019
(This article belongs to the Special Issue Recent Advances in Fusarium Research)
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Abstract

Fungal non-ribosomal peptide synthetase (NRPS) clusters are spread across the chromosomes, where several modifying enzyme-encoding genes typically flank one NRPS. However, a recent study showed that the octapeptide fusaoctaxin A is tandemly synthesized by two NRPSs in Fusarium graminearum. Here, we illuminate parts of the biosynthetic route of fusaoctaxin A, which is cleaved into the tripeptide fusatrixin A and the pentapeptide fusapentaxin A during transport by a cluster-specific ABC transporter with peptidase activity. Further, we deleted the histone H3K27 methyltransferase kmt6, which induced the production of fusaoctaxin A. View Full-Text
Keywords: Fusarium; NRPS; secondary metabolites; Mycotoxins; ABC-transporter; peptidase Fusarium; NRPS; secondary metabolites; Mycotoxins; ABC-transporter; peptidase
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Westphal, K.R.; Nielsen, K.A.H.; Wollenberg, R.D.; Møllehøj, M.B.; Bachleitner, S.; Studt, L.; Lysøe, E.; Giese, H.; Wimmer, R.; Sørensen, J.L.; Sondergaard, T.E. Fusaoctaxin A, an Example of a Two-Step Mechanism for Non-Ribosomal Peptide Assembly and Maturation in Fungi. Toxins 2019, 11, 277.

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