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Open AccessArticle

Toxicity Studies of Chanoclavine in Mice

1
AgResearch Limited, Ruakura Research Centre, Private Bag 3123, Hamilton 3240, New Zealand
2
Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Private Bag 11 222, Palmerston North 4442, New Zealand
*
Author to whom correspondence should be addressed.
Toxins 2019, 11(5), 249; https://doi.org/10.3390/toxins11050249
Received: 16 April 2019 / Revised: 30 April 2019 / Accepted: 30 April 2019 / Published: 2 May 2019
(This article belongs to the Special Issue Biological Activities of Alkaloids: From Toxicology to Pharmacology)
Epichloë endophytes have been used successfully in pastoral grasses providing protection against insect pests through the expression of secondary metabolites. This approach could be extended to other plant species, such as cereals, reducing reliance on pesticides. To be successful, the selected endophyte must express secondary metabolites that are active against cereal insect pests without any secondary metabolite, which is harmful to animals. Chanoclavine is of interest as it is commonly expressed by endophytes and has potential insecticidal activity. Investigation of possible mammalian toxicity is therefore required. An acute oral toxicity study showed the median lethal dose of chanoclavine to be >2000 mg/kg. This allows it to be classified as category 5 using the globally harmonized system of classification and labelling of chemicals, and category 6.1E using the New Zealand Hazardous Substances and New Organisms (HSNO) hazard classes, the lowest hazard class under both systems of classification. A three-week feeding study was also performed, which showed chanoclavine, at a dose rate of 123.9 mg/kg/day, initially reduced food consumption but was resolved by day seven. No toxicologically significant effects on gross pathology, histology, hematology, or blood chemistry were observed. These experiments showed chanoclavine to be of low toxicity and raised no food safety concerns. View Full-Text
Keywords: chanoclavine; toxicology; subchronic feeding study; acute toxicity; endophyte; Epichloë chanoclavine; toxicology; subchronic feeding study; acute toxicity; endophyte; Epichloë
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MDPI and ACS Style

Finch, S.C.; Munday, J.S.; Sprosen, J.M.; Bhattarai, S. Toxicity Studies of Chanoclavine in Mice. Toxins 2019, 11, 249.

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