A Hylarana latouchii Skin Secretion-Derived Novel Bombesin-Related Pentadecapeptide (Ranatensin-HLa) Evoke Myotropic Effects on the in vitro Rat Smooth Muscles
Abstract
:1. Introduction
2. Results
2.1. Bioactivity Screening Resulted in Discovery of Ranatensin-HLa
2.2. Molecular Cloning of the cDNA Encoding the Biosynthetic Precursor of Ranatensin-HLa
2.3. Pharmacological Effects of Ranatensin-HLa on Rat Smooth Muscles
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Specimen Biodata and Skin Secretion Acquisition
5.2. Chromatographic Fractionation of H. latouchii Skin Secretion with Reverse Phase HPLC
5.3. Myoactivity Screening of Chromatographic Fractions
5.4. Structural Characterization of Peptide Possessing Bioactivity
5.5. Molecular Cloning of the cDNA Encoding the Peptide Biosynthetic Precursor of Ranatensin-HLa
5.6. Fmoc Chemistry Solid-Phase Peptide Synthesis of Ranatensin-HLa
5.7. The Effects of Synthetic Peptide on Rat Smooth Muscles Tension
5.8. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Peptides | Bladder (EC50, nM) | Uterus (EC50, nM) |
---|---|---|
Ranatensin-HLa | 7.1 | 5.5 |
Ranatensin-HL | 19.2 | 5.4 |
Ranatensin-HL-10 | 63.8 | 70.9 |
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Lin, Y.; Hu, N.; He, H.; Ma, C.; Zhou, M.; Wang, L.; Chen, T. A Hylarana latouchii Skin Secretion-Derived Novel Bombesin-Related Pentadecapeptide (Ranatensin-HLa) Evoke Myotropic Effects on the in vitro Rat Smooth Muscles. Toxins 2019, 11, 204. https://doi.org/10.3390/toxins11040204
Lin Y, Hu N, He H, Ma C, Zhou M, Wang L, Chen T. A Hylarana latouchii Skin Secretion-Derived Novel Bombesin-Related Pentadecapeptide (Ranatensin-HLa) Evoke Myotropic Effects on the in vitro Rat Smooth Muscles. Toxins. 2019; 11(4):204. https://doi.org/10.3390/toxins11040204
Chicago/Turabian StyleLin, Yan, Nan Hu, Haoyang He, Chengbang Ma, Mei Zhou, Lei Wang, and Tianbao Chen. 2019. "A Hylarana latouchii Skin Secretion-Derived Novel Bombesin-Related Pentadecapeptide (Ranatensin-HLa) Evoke Myotropic Effects on the in vitro Rat Smooth Muscles" Toxins 11, no. 4: 204. https://doi.org/10.3390/toxins11040204
APA StyleLin, Y., Hu, N., He, H., Ma, C., Zhou, M., Wang, L., & Chen, T. (2019). A Hylarana latouchii Skin Secretion-Derived Novel Bombesin-Related Pentadecapeptide (Ranatensin-HLa) Evoke Myotropic Effects on the in vitro Rat Smooth Muscles. Toxins, 11(4), 204. https://doi.org/10.3390/toxins11040204