Proteomic Analysis of Novel Components of Nemopilema nomurai Jellyfish Venom: Deciphering the Mode of Action
Abstract
:1. Introduction
2. Results
2.1. Identification of N. nomurai Nematocyst Proteins by Proteomic Characterization
2.2. Modified Zymography Identify Metalloproteinase and PLA2 in NnV
2.3. Ontological Classification of Differentially Expressed Proteins
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Chemicals and Reagents
5.2. Sample Collection and Preparation
5.3. Venom Extraction
5.4. Two-Dimensional Gel Electrophoresis under Reducing Conditions and Image Analysis
5.5. In-gel Digestion
5.6. MALDI/TOF/MS Analysis and Database Searching
5.7. Proteolytic Activity Assay
5.8. Modified Zymography Assays to Identify PLA2 Activity in NnV
5.9. Two-Dimensional Gel Electrophoresis under Non-Reducing Conditions and 2-DE Gelatin Zymography
5.10. Gene Ontology Analysis of the Identified Venom Proteins
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Spot No | Accession Number 1 | Protein Name | Uniprot ID | Therotical MW/Pi 2 | Organism | Matched Peptide 3 | MOWSE Score | Biological Process |
---|---|---|---|---|---|---|---|---|
1450 | P23897 | Heat-stable enterotoxin recepter | GUC2C_RAT | 123,468/6.4 | Rattus norvegicus | 8% | 4851 | Intracellular signal, transduction, regulation of cell proliferation |
1053 | P55128 | RTX-I toxin determinant A from serotypes 1/9 | RTXII_ACTPL | 110,194/5.5 | Actinobacillus pleuropneumoniae | 9.20% | 4636 | Hemolysis in other organism, pathogenesis |
1622 | P15321 | Hemolysin transporter, protein ShlB | HLYB_SERMA | 61,917/9.2 | Serratia marcescens | 15.30% | 3414 | Hemolysis in other organism, pathogenesis protein transmembrane transport |
1260 | Q1W694 | Phospholipase D LiSicTox-betaIDI | B1Q_LOXIN | 34,831/7.6 | Loxosceles intermedia | 22.00% | 154 | Pathogenesis, Hemolysis in other organism, phospholipid catabolic process |
1338 | P39673 | Allergen Mag (fragment) | MAG_DERFA | 39,668/6.9 | Dermatophagoides farinae | 11.70% | 320 | |
721 | P55123 | Leukotoxin | LKTA_PASSP | 101,560/5.6 | Pasteurella haemo-lytica-like sp. (strain 5943b) | 8.40% | 1331 | Hemolysis in other organism, pathogenesis |
1213 | B2BS84 | Putative Kunitz-type serine protease inhibitor | VKT_AUSLA | 27,571/7.9 | Austrelaps labialis | 25.00% | 7.14 × 102 | |
1486 | P0C845 | Turripeptide Gsp9.1 | C91_GEMSP | 9290/9.1 | Gemmula speciosa | 26.80% | 1802 | |
837 | F5CPD3 | Three-finger toxin MALT0044C | 3SX4_MICAT | 9398/8.4 | Micrurus altirostris | 54.10% | 8534 | Pathogenesis |
1643 | P81428 | Venom prothrombin activator trocarin-D | FAXD_TROCA | 51,407/8.1 | Tropidechis carinatus | 9.90% | 294 | Blood coagulation, envenomation resulting in positive regulation of blood coagulation in other organism |
889 | P55130 | RTX-III toxin determinant A from serotype 2 | RTX31_ACTPL | 112,492/5.8 | Actinobacillus pleuropneumoniae | 13.00% | 6.89 × 106 | Cytolysis, pathogenesis |
930 | O59824 | ATP-dependent zinc metalloprotease YME1 homolog | YME1_SCHPO | 78,219/8.5 | Schizosaccharomyces pombe (strain 972/ATCC 24843) | 6.20% | 1335 | proteolysis |
1614 | Q7S9D2 | Pro-apoptotic serine protease nma111 | NM111_NEUCR | 113,312/5.7 | Neurospora crassa | 10.80% | 30913 | Apoptotic process |
1182 | P08026 | Shiga-like toxin 1 subunit A | STXA_BPH19 | 34,800/9.6 | Enterobacteria phage H19B | 21% | 2329 | negative regulation of translation, pathogenesis |
1450 | Q2FZP2 | Serine protease HtrA-like | HTRAL_STAA8 | 86,460/6.5 | Staphylococcus aureus (strain NCTC 8325) | 20.20% | 876,777 | |
1647 | E5AJX2 | Snake venom serine protease nikobin | VSP_VIPNI | 28,216/8.0 | Vipera nikolskii | 18.20% | 425 | |
801 | Q2QA02 | Zinc metalloproteinase-disintegrin-like alternative name Snake venom metalloproteinase | VM3_CRODD | 68,292/5.1 | Crotalus durissus durissus | 19.50% | 57,907 | |
1233 | Q40240 | Major pollen allergen Lol p 5a | MPA5A_LOLPR | 30,888/5.4 | Lolium perenne | 16.90% | 707 | type I hypersensitivity |
1339 | P54319 | Phospholipase A-2-activating protein | PLAP_RAT | 87,085/5.7 | Rattus norvegicus | 11.30% | 1961 | Inflammatory response |
1343 | P23636 | Major serine/threonine-protein phosphatase PP2A_2 catalytic subunit | PP2A2_SCHPO | 36,489/4.7 | Schizosaccharomyces pombe (strain 972/ATCC 24843) | 19.60% | 2620 | Cell division, signal transduction, mitotic nuclear division |
1206 | Q9TT93 | A disintegrin and metallo-proteinase with thrombo-spondin motifs 4 | ATS4_BOVIN | 90,281/8.6 | Bos taurus | 11.90% | 3.76 × 104 | Proteolysis, Angiogenesis |
1500 | P97570 | 85/88 kDa calcium-independent phospholipase A2 | PLPL_RAT | 89,556/6.7 | Rattus norvegicus | 12% | 19,976 | ATP dependent protein binding, chemotaxis, positive regulation of vasodilation |
1315 | P58459 | A disintegrin and metalloproteinase with thrombospondin motifs 10 | ATS10_MOUSE | 121,087/8.4 | Mus musculus | 11.30% | 40,176 | Microfibrils assembly |
1382 | Q9R1V7 | A disintegrin and metalloproteinase with thrombospondin motifs 23 | ADA_23 MOUSE | 91,548/7.9 | Mus musculus | 14% | 2773 | Cell adhesion |
1461 | Q9XWD6 | Cell death abnormality protein 1 | CED1_CAEEL | 118,805/5.5 | Caenorhabditis elegans | 10.10% | 6333 | Programmed cell death, apoptotic cell death, receptor-mediated endocytosis, |
1272 | Q9T051 | Phospholipase D gamma 2 | PLDG2_ARATH | 96,024/8.3 | Arabidopsis thaliana | 12.60% | 11,703 | Response to stress, membrane lipid metabolic process, phosphotidylcholine metabolic process |
1334 | Q10743 | A disintegrin and metalloproteinase with thrombospondin motifs 10 fragment | ADA10_RAT | 60,445/8.4 | Rattus norvegicus | 15.10% | 5386 | Negative regulation of cell adhesion, Notch signaling pathway, protein phosphorylation |
1498 | Q83XX3 | ATP-dependent zinc metalloprotease FtsH | FTSH_OENOE | 78,070/9.3 | Oenococcus oeni | 10.30% | 447 | Protein catabolic process |
867 | P78536 | Disintegrin and metalloproteinase domain-containing protein 17 | ADA17_HUMAN | 93,022/5.5 | Homo sapiens | 10.70% | 2397 | Positive regulation of cell growth and cell migration, negative regulation of transforming growth factor beta receptor signaling |
1434 | A8XEZ1 | Cell death abnormality protein 12 | CED12_CAEBR | 83,692/5.2 | Saccharomyces cerevisiae (strain ATCC 204508/S288c) | 9.00% | 3371 | Engulfment of apoptotic cell, cell migration, apoptotic process, phagocytosis, positive regulation of GTPase activity |
1558 | Q9S5Z2 | ATP-dependent Clp protease ATP-binding subunit ClpE | PLPL8_MOUSE | 87,382/9.3 | Mus musculus | 9.10% | 2564 | Cell death, arachidonic acid, secretion, phosphatidylcholine catabolic process |
1418 | Q8K1N1 | Calcium-independent phospholipase A2-gamma | HTRAL_STAA8 | 86,460/6.5 | Staphylococcus aureus (strain NCTC 8325) | 20.20% | 876,777 | |
1183 | O93654 | Tricorn protease-interacting factor F2 | NAS8_CAEEL | 46,096/5.8 | Caenorhabditis elegans | 13.40% | 104 | |
1087 | Q18439 | Zinc metalloproteinase nas-8 | CYM1_YEAST | 112,181/6.0 | Saccharomyces cerevisiae (strain ATCC 204508/S288c) | 18.20% | 383,182 | Protein processing, proteolysis, 54 |
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Choudhary, I.; Hwang, D.H.; Lee, H.; Yoon, W.D.; Chae, J.; Han, C.H.; Yum, S.; Kang, C.; Kim, E. Proteomic Analysis of Novel Components of Nemopilema nomurai Jellyfish Venom: Deciphering the Mode of Action. Toxins 2019, 11, 153. https://doi.org/10.3390/toxins11030153
Choudhary I, Hwang DH, Lee H, Yoon WD, Chae J, Han CH, Yum S, Kang C, Kim E. Proteomic Analysis of Novel Components of Nemopilema nomurai Jellyfish Venom: Deciphering the Mode of Action. Toxins. 2019; 11(3):153. https://doi.org/10.3390/toxins11030153
Chicago/Turabian StyleChoudhary, Indu, Du Hyeon Hwang, Hyunkyoung Lee, Won Duk Yoon, Jinho Chae, Chang Hoon Han, Seungshic Yum, Changkeun Kang, and Euikyung Kim. 2019. "Proteomic Analysis of Novel Components of Nemopilema nomurai Jellyfish Venom: Deciphering the Mode of Action" Toxins 11, no. 3: 153. https://doi.org/10.3390/toxins11030153