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Toxins 2019, 11(3), 143; https://doi.org/10.3390/toxins11030143

Molecular Interaction-Based Exploration of the Broad Spectrum Efficacy of a Bacillus thuringiensis Insecticidal Chimeric Protein, Cry1AcF

1
ICAR-National Research Centre on Plant Biotechnology, New Delhi 110012, India
2
Division of Biochemistry, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India
*
Author to whom correspondence should be addressed.
Received: 28 December 2018 / Revised: 4 February 2019 / Accepted: 6 February 2019 / Published: 2 March 2019
(This article belongs to the Special Issue Insecticidal Toxins from Bacillus thuringiensis)
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Abstract

Bacillus thuringiensis insecticidal proteins (Bt ICPs) are reliable and valuable options for pest management in crops. Protein engineering of Bt ICPs is a competitive alternative for resistance management in insects. The primary focus of the study was to reiterate the translational utility of a protein-engineered chimeric Cry toxin, Cry1AcF, for its broad spectrum insecticidal efficacy using molecular modeling and docking studies. In-depth bioinformatic analysis was undertaken for structure prediction of the Cry toxin as the ligand and aminopeptidase1 receptors (APN1) from Helicoverpa armigera (HaAPN1) and Spodoptera litura (SlAPN1) as receptors, followed by interaction studies using protein-protein docking tools. The study revealed feasible interactions between the toxin and the two receptors through H-bonding and hydrophobic interactions. Further, molecular dynamics simulations substantiated the stability of the interactions, proving the broad spectrum efficacy of Cry1AcF in controlling H. armigera and S. litura. These findings justify the utility of protein-engineered toxins in pest management. View Full-Text
Keywords: chimeric protein; insecticidal proteins; insect resistance; modeling; protein-protein docking chimeric protein; insecticidal proteins; insect resistance; modeling; protein-protein docking
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Rathinam, M.; Kesiraju, K.; Singh, S.; Thimmegowda, V.; Rai, V.; Pattanayak, D.; Sreevathsa, R. Molecular Interaction-Based Exploration of the Broad Spectrum Efficacy of a Bacillus thuringiensis Insecticidal Chimeric Protein, Cry1AcF. Toxins 2019, 11, 143.

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