Evaluation of the Liver Toxicity of Pterocephalus hookeri Extract via Triggering Necrosis
AbstractPterocephalus hookeri (C. B. Clarke) Höeck, recorded in the Chinese Pharmacopoeia (2015 version) as a Tibetan medicine for the treatment of various diseases, especially rheumatoid arthritis, was believed to possess a slight toxicity. However, hardly any research has been carried out about it. The present study aimed to evaluate the toxicity in vivo and in vitro. Toxicity was observed by the evaluation of mice weight loss and histopathological changes in the liver. Then, the comparison research between ethyl acetate extract (EAE) and n-butanol extract (BUE) suggested that liver toxicity was mainly induced by BUE. The mechanical study suggested that BUE-induced liver toxicity was closely associated with necrosis detected by MTT and propidium iodide (PI) staining, via releasing lactate dehydrogenase (LDH), reducing the fluidity, and increasing the permeability of the cell membrane. Western blot analysis confirmed that the necrosis occurred molecularly by the up-regulation of receptor-interacting protein kinase 1 (RIP1) and receptor-interacting protein kinase 3 (RIP3), as well as the activation of the nuclear factor-kappa-gene binding (NF-κB) signaling pathway in vivo and in vitro. This finding indicated that the liver toxicity induced by BUE from P. hookeri was mainly caused by necrosis, which provides an important theoretical support for further evaluation of the safety of this folk medicine. View Full-Text
- Supplementary File 1:
PDF-Document (PDF, 145 KB)
Share & Cite This Article
Wang, R.; Dong, Z.; Zhang, X.; Mao, J.; Meng, F.; Lan, X.; Liao, Z.; Chen, M. Evaluation of the Liver Toxicity of Pterocephalus hookeri Extract via Triggering Necrosis. Toxins 2019, 11, 142.
Wang R, Dong Z, Zhang X, Mao J, Meng F, Lan X, Liao Z, Chen M. Evaluation of the Liver Toxicity of Pterocephalus hookeri Extract via Triggering Necrosis. Toxins. 2019; 11(3):142.Chicago/Turabian Style
Wang, Rui; Dong, Zhaoyue; Zhang, Xiaolong; Mao, Jingxin; Meng, Fancheng; Lan, Xiaozhong; Liao, Zhihua; Chen, Min. 2019. "Evaluation of the Liver Toxicity of Pterocephalus hookeri Extract via Triggering Necrosis." Toxins 11, no. 3: 142.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.