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Open AccessArticle

The Effect of a Combined Hydrogen Peroxide-MlrA Treatment on the Phytoplankton Community and Microcystin Concentrations in a Mesocosm Experiment in Lake Ludoš

1
Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30387 Krakow, Poland
2
Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovića 2, 21000 Novi Sad, Serbia
3
Department of Hydrobiology, Faculty of Biology, Adam Mickiewicz University, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
4
Department of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30387 Krakow, Poland
5
Biochemistry, Faculty of Science and Engineering, Åbo Akademi University, Tykistökatu 6 A, 20520 Turku, Finland
*
Author to whom correspondence should be addressed.
Toxins 2019, 11(12), 725; https://doi.org/10.3390/toxins11120725
Received: 31 October 2019 / Revised: 2 December 2019 / Accepted: 5 December 2019 / Published: 11 December 2019
Harmful cyanobacteria and their toxic metabolites constitute a big challenge for the production of safe drinking water. Microcystins (MC), chemically stable hepatotoxic heptapeptides, have often been involved in cyanobacterial poisoning incidents. A desirable solution for cyanobacterial management in lakes and ponds would eliminate both excess cyanobacteria and the MC that they potentially produce and release upon lysis. Hydrogen peroxide (H2O2) has recently been advocated as an efficient means of lysing cyanobacteria in lakes and ponds, however H2O2 (at least when used at typical concentrations) cannot degrade MC in environmental waters. Therefore, mesocosm experiments combining the cyanobacteria-lysing effect of H2O2 and the MC-degrading capacity of the enzyme MlrA were set up in the highly eutrophic Lake Ludoš (Serbia). The H2O2 treatment decreased the abundance of the dominant cyanobacterial taxa Limnothrix sp., Aphanizomenon flos-aquae, and Planktothrix agardhii. The intracellular concentration of MC was reduced/eliminated by H2O2, yet the reduction of the extracellular MC could only be accomplished by supplementation with MlrA. However, as H2O2 was found to induce the expression of mcyB and mcyE genes, which are involved in MC biosynthesis, the use of H2O2 as a safe cyanobacteriocide still requires further investigation. In conclusion, the experiments showed that the combined use of H2O2 and MlrA is promising in the elimination of both excess cyanobacteria and their MC in environmental waters. View Full-Text
Keywords: microcystin; biodegradation; hydrogen peroxide; treatment; cyanobacteria; bloom microcystin; biodegradation; hydrogen peroxide; treatment; cyanobacteria; bloom
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Dziga, D.; Tokodi, N.; Drobac, D.; Kokociński, M.; Antosiak, A.; Puchalski, J.; Strzałka, W.; Madej, M.; Svirčev, Z.; Meriluoto, J. The Effect of a Combined Hydrogen Peroxide-MlrA Treatment on the Phytoplankton Community and Microcystin Concentrations in a Mesocosm Experiment in Lake Ludoš. Toxins 2019, 11, 725.

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