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Open AccessCommunication

Graphene-Based Sensing Platform for On-Chip Ochratoxin A Detection

1
National Research University of Electronic Technology, Zelenograd, 124498 Moscow, Russia
2
Department of Electrical and Computer Engineering, The University of Texas at Austin, Austin, TX 78712, USA
3
P.N. Lebedev Physical Institute of the Russian Academy of Sciences, 119991 Moscow, Russia
4
BioSense Institute—Research and Development Institute for Information Technologies in Biosystems, University of Novi Sad, 21000 Novi Sad, Serbia
*
Author to whom correspondence should be addressed.
Toxins 2019, 11(10), 550; https://doi.org/10.3390/toxins11100550
Received: 15 August 2019 / Revised: 12 September 2019 / Accepted: 18 September 2019 / Published: 20 September 2019
(This article belongs to the Special Issue Advanced Methods for Mycotoxins Detection)
In this work, we report an on-chip aptasensor for ochratoxin A (OTA) toxin detection that is based on a graphene field-effect transistor (GFET). Graphene-based devices are fabricated via large-scale technology, allowing for upscaling the sensor fabrication and lowering the device cost. The sensor assembly was performed through covalent bonding of graphene’s surface with an aptamer specifically sensitive towards OTA. The results demonstrate fast (within 5 min) response to OTA exposure with a linear range of detection between 4 ng/mL and 10 pg/mL, with a detection limit of 4 pg/mL. The regeneration time constant of the sensor was found to be rather small, only 5.6 s, meaning fast sensor regeneration for multiple usages. The high reproducibility of the sensing response was demonstrated via using several recycling procedures as well as various GFETs. The applicability of the aptasensor to real samples was demonstrated for spiked red wine samples with recovery of about 105% for a 100 pM OTA concentration; the selectivity of the sensor was also confirmed via addition of another toxin, zearalenone. The developed platform opens the way for multiplex sensing of different toxins using an on-chip array of graphene sensors. View Full-Text
Keywords: graphene; transistor; ochratoxin A; aptamer; sensor; on-chip graphene; transistor; ochratoxin A; aptamer; sensor; on-chip
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MDPI and ACS Style

Nekrasov, N.; Kireev, D.; Emelianov, A.; Bobrinetskiy, I. Graphene-Based Sensing Platform for On-Chip Ochratoxin A Detection. Toxins 2019, 11, 550.

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