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Toxins 2019, 11(1), 56; https://doi.org/10.3390/toxins11010056

Simultaneous Lateral Flow Immunoassay for Multi-Class Chemical Contaminants in Maize and Peanut with One-Stop Sample Preparation

1,2,†
,
1,†
,
1,* , 1,3,4,* , 1,2,3,* , 1,3
,
1,3
,
1,3
,
1,3
and
1,3,4,5,*
1
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China
2
Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China
3
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China
4
Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China
5
Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture and Rural Affairs, Wuhan 430062, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 26 December 2018 / Revised: 17 January 2019 / Accepted: 17 January 2019 / Published: 20 January 2019
(This article belongs to the Special Issue Advanced Methods for Mycotoxins Detection)
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Abstract

Multi-class chemical contaminants, such as pesticides and mycotoxins, are recognized as the major risk factors in agro products. It is thus necessary to develop rapid and simple sensing methods to fulfill the on-site monitoring of multi-class chemical contaminants with different physicochemical properties. Herein, a lateral flow immunoassay via time-resolved fluorescence was developed for the rapid, on-site, simultaneous, and quantitative sensing aflatoxin B1 (AFB1), zearalenone (ZEA), and chlorothalonil (CTN) in maize and peanut. The sample preparation was optimized to a single step, combining the grinding and extraction. Under optimal conditions, the sensing method lowered the limits of detection (LOD) to 0.16, 0.52, and 1.21 µg/kg in maize and 0.18, 0.57, and 1.47 µg/kg in peanut with an analytical range of 0.48–20, 1.56–200, and 3.63–300 µg/kg for AFB1, ZEA and CTN, respectively. The protocol could be completed within 15 min, including sample preparation and lateral flow immunoassay. The recovery range was 83.24–110.80%. An excellent correlation was observed between this approach and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for mycotoxins and gas chromatography-tandem mass spectrometry (GC-MS/MS) for pesticide in maize and peanut. This work could be applied in on-site multi-class sensing for food safety. View Full-Text
Keywords: simultaneous lateral flow immunoassay; multi-class chemical contaminants; aflatoxin B1; zearalenone; chlorothalonil; maize and peanut simultaneous lateral flow immunoassay; multi-class chemical contaminants; aflatoxin B1; zearalenone; chlorothalonil; maize and peanut
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Wang, D.; Zhu, J.; Zhang, Z.; Zhang, Q.; Zhang, W.; Yu, L.; Jiang, J.; Chen, X.; Wang, X.; Li, P. Simultaneous Lateral Flow Immunoassay for Multi-Class Chemical Contaminants in Maize and Peanut with One-Stop Sample Preparation. Toxins 2019, 11, 56.

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