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Antibody Cross-Reactivity in Antivenom Research
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Toxins 2019, 11(1), 32; https://doi.org/10.3390/toxins11010032

Generation of a Broadly Cross-Neutralizing Antibody Fragment against Several Mexican Scorpion Venoms

1
CONACYT, Instituto de Biotecnología—Universidad Nacional Autónoma de México, Apartado Postal 510-3, Cuernavaca, Morelos 62250, Mexico
2
Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, UNAM, Apartado Postal 510-3, Cuernavaca, Morelos 62250, Mexico
*
Author to whom correspondence should be addressed.
Received: 11 December 2018 / Revised: 3 January 2019 / Accepted: 3 January 2019 / Published: 10 January 2019
(This article belongs to the Special Issue Discovery of Antibodies and Novel Antivenoms against Envenoming)
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Abstract

The recombinant antibody fragments generated against the toxic components of scorpion venoms are considered a promising alternative for obtaining new antivenoms for therapy. Using directed evolution and site-directed mutagenesis, it was possible to generate a human single-chain antibody fragment with a broad cross-reactivity that retained recognition for its original antigen. This variant is the first antibody fragment that neutralizes the effect of an estimated 13 neurotoxins present in the venom of nine species of Mexican scorpions. This single antibody fragment showed the properties of a polyvalent antivenom. These results represent a significant advance in the development of new antivenoms against scorpion stings, since the number of components would be minimized due to their broad cross-neutralization capacity, while at the same time bypassing animal immunization. View Full-Text
Keywords: cross-neutralization; directed evolution; phage display; polyvalent antivenom; scFv; scorpion cross-neutralization; directed evolution; phage display; polyvalent antivenom; scFv; scorpion
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Riaño-Umbarila, L.; Gómez-Ramírez, I.V.; Ledezma-Candanoza, L.M.; Olamendi-Portugal, T.; Rodríguez-Rodríguez, E.R.; Fernández-Taboada, G.; Possani, L.D.; Becerril, B. Generation of a Broadly Cross-Neutralizing Antibody Fragment against Several Mexican Scorpion Venoms. Toxins 2019, 11, 32.

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