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Open AccessArticle

OTA Prevention and Detoxification by Actinobacterial Strains and Activated Carbon Fibers: Preliminary Results

Laboratoire de Génie Chimique, Université de Toulouse, CNRS, Toulouse, France; Avenue de l’Agrobiopole-BP 32607-Auzeville-Tolosane 31326 CASTANET-TOLOSAN CEDEX
Unité de Recherche Biologie des Plantes et Innovation (BIOPI-EA 3900), Université de Picardie Jules Verne, 33 rue Saint Leu, 80039 Amiens CEDEX, France
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Toxins 2018, 10(4), 137;
Received: 16 March 2018 / Revised: 20 March 2018 / Accepted: 22 March 2018 / Published: 24 March 2018
(This article belongs to the Collection Ochratoxins-Collection)
Ochratoxin A (OTA) is a mycotoxin produced by several species of Aspergillus and Penicillium that contaminate food and feed raw materials. To reduce OTA contamination, we first tested in vitro, actinobacterial strains as potential biocontrol agents and afterward, through a physical decontamination method using activated carbon fibers (ACFs). Actinobacterial strains were screened for their ability to reduce OTA in solid co-culture with A. carbonarius, which is the major OTA-producing species in European vineyards. Four strains showed a high affinity for removing OTA (67%–83%) with no significant effect on fungal growth (<20%). The mechanism of action was first studied by analyzing the expression of OTA cluster genes (acOTApks, acOTAnrps, acOTAhal) by RT-qPCR showing a drastic reduction in all genes (7–15 times). Second, the ability of these strains to degrade OTA was assessed in vitro on ISP2 solid medium supplemented with OTA (100 µg/L). Two strains reduced OTA to undetectable levels. As for the physical method, high adsorption rates were obtained for ACFs at 0.8 g/L with a 50% adsorption of OTA in red wine by AC15 and 52% in grape juice by AC20 within 24 h. These promising methods could be complementarily applied toward reducing OTA contamination in food chains, which promotes food safety and quality. View Full-Text
Keywords: ochratoxin A; biocontrol; detoxification; actinobacteria; activated carbon fibers ochratoxin A; biocontrol; detoxification; actinobacteria; activated carbon fibers
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El Khoury, R.; Choque, E.; El Khoury, A.; Snini, S.P.; Cairns, R.; Andriantsiferana, C.; Mathieu, F. OTA Prevention and Detoxification by Actinobacterial Strains and Activated Carbon Fibers: Preliminary Results. Toxins 2018, 10, 137.

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