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Toxins 2018, 10(3), 122; https://doi.org/10.3390/toxins10030122

Analysing the Structural Effect of Point Mutations of Cytotoxic Necrotizing Factor 1 (CNF1) on Lu/BCAM Adhesion Glycoprotein Association

Univ Paris Diderot, Sorbonne Paris Cite, Univ de la Réunion, Univ des Antilles, Inserm UMR_S 1134, INTS, Laboratoire d’Excellence GR-Ex, 75015 Paris, France
Received: 28 February 2018 / Revised: 6 March 2018 / Accepted: 8 March 2018 / Published: 13 March 2018
(This article belongs to the Section Bacterial Toxins)
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Abstract

Cytotoxic Necrotizing Factor 1 (CNF1) was identified in 1983 as a protein toxin produced by certain pathogenic strains of Escherichia coli. Since then, numerous studies have investigated its particularities. For instance, it is associated with the single chain AB-toxin family, and can be divided into different functional and structural domains, e.g., catalytic and transmembrane domain and interaction sites. A few years ago, the identification of the Lutheran (Lu) adhesion glycoprotein/basal cell adhesion molecule (BCAM) as a cellular receptor for CNF1 provided new insights into the adhesion process of CNF1. Very recently, the Ig-like domain 2 of Lu/BCAM was confirmed as the main interaction site using protein-protein interaction and competition studies with various different mutants. Here, I present in silico approaches that precisely explain the impact of these mutations, leading to a better explanation of these experimental studies. These results can be used in the development of future antitoxin strategies. View Full-Text
Keywords: Lu/BCAM; CNF; laminin; toxin; receptor; immunoglobulin-like domain; in silico approaches; computational biology; side-chains; protein-protein interaction; sickle cell disease Lu/BCAM; CNF; laminin; toxin; receptor; immunoglobulin-like domain; in silico approaches; computational biology; side-chains; protein-protein interaction; sickle cell disease
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de Brevern, A.G. Analysing the Structural Effect of Point Mutations of Cytotoxic Necrotizing Factor 1 (CNF1) on Lu/BCAM Adhesion Glycoprotein Association. Toxins 2018, 10, 122.

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