Dietary Fructose Enhances the Ability of Low Concentrations of Angiotensin II to Stimulate Proximal Tubule Na+ Reabsorption
Abstract
:1. Introduction
2. Materials and Methods
3. Results
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Bicarbonate- Buffered Physiological Saline | HEPES- Buffered Physiological Saline | K-Free HEPES-Buffered Solution | Acid Pulse Buffer | 4X Reaction Media | 4X Reaction Media with Ouabain | ||
---|---|---|---|---|---|---|---|
NaHCO3 | (mmol/L) | 25.0 | - | - | - | - | - |
HEPES | " | - | 10.0 | 10.0 | 10.0 | - | - |
Imidazole | " | - | - | - | - | 200.0 | 200.0 |
NaCl | " | 114.0 | 130.0 | 130.0 | 120.0 | 320.0 | 320.0 |
KCl | " | 4.0 | 4.0 | - | 4.0 | 120.0 | - |
Na2HPO4 | " | 2.1 | 2.5 | 2.5 | 2.5 | - | - |
NaH2PO4 | " | 0.4 | - | - | - | - | - |
Mg SO4 | " | 1.2 | 1.2 | 1.2 | 1.2 | 20.0 | 20.0 |
Ca(Lactate)2 | " | 2.0 | 2.0 | 2.0 | 2.0 | - | - |
Na3Citrate | " | 1.0 | 1.0 | 1.0 | 1.0 | - | - |
DL-alanine | " | 6.0 | 6.0 | 6.0 | 6.0 | - | - |
Glucose | " | 5.5 | 5.5 | 5.5 | 5.5 | - | - |
NH4Cl | " | - | - | - | 10.0 | - | - |
EGTA | " | - | - | - | - | 2.0 | 2.0 |
Na2ATP | " | - | - | - | - | 20.0 | 20.0 |
NADH | " | - | - | - | - | 4.0 | 4.0 |
Ascorbic Acid | " | - | - | - | - | 4.0 | 4.0 |
PEP | " | - | - | - | - | 40.0 | 40.0 |
Antibody | Provider | Catalog | Source | Blocking | Conditions | ||
---|---|---|---|---|---|---|---|
Number | Buffer | Dilution | Buffer | Time | |||
NHE3 | Abcam | ab95299 | Rabbit | 5% BSA | 1:1000 | 5% BSA | 2 h |
α1-Na/K-ATPase | Cell Signaling | #3010 | Rabbit | 5% Milk | 1:5000 | 5% Milk | 2 h |
β-tubulin | Abcam | ab6046 | Rabbit | 5% Milk | 1:10,000 | 5% Milk | 2 h |
GAPDH-HRP | Abcam | ab9485 | - | 5% BSA | 1:15,000 | 5% BSA | 2 h |
2ry anti-Rabbit-HRP | GE Healthcare | NA9340V | Donkey | - | 1:2500 | 5% BSA | 1 h |
Control (n = 5) | Fructose (n = 6) | Change | T test | ||||
Mean | SEM | Mean | SEM | ||||
Caloric Intake | (kcal/24 h) | 69.9 | 6.0 | 65.5 | 3.2 | = | p < 0.51 |
Weight Gain | (g/24 h) | 9.4 | 1.3 | 9.2 | 2.4 | = | p < 0.94 |
Fluid Intake | (mL/24 h) | 29.2 | 4.6 | 25.8 | 3.1 | = | p < 0.55 |
Food Intake | (g/24 h) | 17.4 | 1.5 | 12.0 | 0.5 | ↓ | p < 0.01 |
Final Weight | (g) | 236 | 7 | 232 | 10 | = | p < 0.72 |
Systolic BP | (mmHg) | 130 | 11 | 147 | 6 | = | p < 0.18 |
Control | Fructose | Change | T test | ||||
Mean | SEM | Mean | SEM | ||||
pH | 7.42 | 0.01 | 7.38 | 0.03 | = | p < 0.18 | |
Na | (mmol/L) | 136.6 | 0.6 | 137.5 | 0.5 | = | p < 0.31 |
K | (mmol/L) | 3.8 | 0.2 | 3.7 | 0.1 | = | p < 0.73 |
Cl | (mmol/L) | 106.4 | 0.5 | 106.0 | 0.3 | = | p < 0.55 |
Lactate | (mmol/L) | 1.02 | 0.14 | 1.03 | 0.07 | = | p < 0.98 |
Insulin | (µg/mL) | 0.38 | 0.14 | 0.36 | 0.15 | = | p < 0.93 |
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Gonzalez-Vicente, A.; Cabral, P.D.; Hong, N.J.; Asirwatham, J.; Yang, N.; Berthiaume, J.M.; Dominici, F.P.; Garvin, J.L. Dietary Fructose Enhances the Ability of Low Concentrations of Angiotensin II to Stimulate Proximal Tubule Na+ Reabsorption. Nutrients 2017, 9, 885. https://doi.org/10.3390/nu9080885
Gonzalez-Vicente A, Cabral PD, Hong NJ, Asirwatham J, Yang N, Berthiaume JM, Dominici FP, Garvin JL. Dietary Fructose Enhances the Ability of Low Concentrations of Angiotensin II to Stimulate Proximal Tubule Na+ Reabsorption. Nutrients. 2017; 9(8):885. https://doi.org/10.3390/nu9080885
Chicago/Turabian StyleGonzalez-Vicente, Agustin, Pablo D. Cabral, Nancy J. Hong, Jessica Asirwatham, Nianxin Yang, Jessica M. Berthiaume, Fernando P. Dominici, and Jeffrey L. Garvin. 2017. "Dietary Fructose Enhances the Ability of Low Concentrations of Angiotensin II to Stimulate Proximal Tubule Na+ Reabsorption" Nutrients 9, no. 8: 885. https://doi.org/10.3390/nu9080885