Sweet Taste Receptor Activation in the Gut Is of Limited Importance for Glucose-Stimulated GLP-1 and GIP Secretion
Abstract
:1. Introduction
2. Materials and Methods
2.1. Perfusion of the Proximal Small Intestine
2.2. Perfusion Protocol and Test Substances
2.3. Hormone Secretion Analysis
2.4. Statistical Analysis
3. Results
3.1. Glucose-Induced Incretin Secretion
3.1.1. Glucose Stimulates GLP-1 and GIP Secretion from the Perfused Rat Small Intestine
3.1.2. Inhibition of the Murine Sweet Taste Receptor Does Not Change Glucose-Induced GLP-1 and GIP Secretion
3.2. Arificial Sweeteners and Incretin Secretion
3.2.1. Luminal Administration of Acesulfame K, but Not Sucralose or Stevioside, Stimulates GLP-1, but Not GIP Secretion
3.2.2. Vascular Administration of Sucralose and Stevioside, but Not Acesulafme K, Stimulates GLP-1 and GIP Secretion
3.2.3. Sucralose Only Stimulates GLP-1 Secretion at High Doses
3.2.4. Inhibition of the Murine Sweet Taste Receptor Does Not Eliminate Sucralose-Induced GLP-1 secretion
3.3. Inhibition of TRPM5-Channel Does Not Attenuate Glucose or Sucralose-Stimulated GLP-1 Secretion
3.3.1. Glucose-Induced GLP-1 Secretion Was not Reduced, but Increased, by Inhibition of TRPM5 Channels
3.3.2. Sucralose-Induced GLP-1 Secretion Is not Changed by Inhibition of TRPM5 Channels
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Saltiel, M.Y.; Kuhre, R.E.; Christiansen, C.B.; Eliasen, R.; Conde-Frieboes, K.W.; Rosenkilde, M.M.; Holst, J.J. Sweet Taste Receptor Activation in the Gut Is of Limited Importance for Glucose-Stimulated GLP-1 and GIP Secretion. Nutrients 2017, 9, 418. https://doi.org/10.3390/nu9040418
Saltiel MY, Kuhre RE, Christiansen CB, Eliasen R, Conde-Frieboes KW, Rosenkilde MM, Holst JJ. Sweet Taste Receptor Activation in the Gut Is of Limited Importance for Glucose-Stimulated GLP-1 and GIP Secretion. Nutrients. 2017; 9(4):418. https://doi.org/10.3390/nu9040418
Chicago/Turabian StyleSaltiel, Monika Y., Rune E. Kuhre, Charlotte B. Christiansen, Rasmus Eliasen, Kilian W. Conde-Frieboes, Mette M. Rosenkilde, and Jens J. Holst. 2017. "Sweet Taste Receptor Activation in the Gut Is of Limited Importance for Glucose-Stimulated GLP-1 and GIP Secretion" Nutrients 9, no. 4: 418. https://doi.org/10.3390/nu9040418