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Nutrients 2017, 9(4), 323;

Fructose-Rich Diet Affects Mitochondrial DNA Damage and Repair in Rats

Department of Science and Technology, University of Sannio, 82100 Benevento, Italy
Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Naples II, 81100 Caserta, Italy
Department of Biology, University of Naples “Federico II”, 80100 Napoli, Italy
Author to whom correspondence should be addressed.
Received: 23 February 2017 / Revised: 17 March 2017 / Accepted: 23 March 2017 / Published: 24 March 2017
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Evidence indicates that many forms of fructose-induced metabolic disturbance are associated with oxidative stress and mitochondrial dysfunction. Mitochondria are prominent targets of oxidative damage; however, it is not clear whether mitochondrial DNA (mtDNA) damage and/or its lack of repair are events involved in metabolic disease resulting from a fructose-rich diet. In the present study, we evaluated the degree of oxidative damage to liver mtDNA and its repair, in addition to the state of oxidative stress and antioxidant defense in the liver of rats fed a high-fructose diet. We used male rats feeding on a high-fructose or control diet for eight weeks. Our results showed an increase in mtDNA damage in the liver of rats fed a high-fructose diet and this damage, as evaluated by the expression of DNA polymerase γ, was not repaired; in addition, the mtDNA copy number was found to be significantly reduced. A reduction in the mtDNA copy number is indicative of impaired mitochondrial biogenesis, as is the finding of a reduction in the expression of genes involved in mitochondrial biogenesis. In conclusion, a fructose-rich diet leads to mitochondrial and mtDNA damage, which consequently may have a role in liver dysfunction and metabolic diseases. View Full-Text
Keywords: fructose-rich diet; mitochondrial biogenesis; mitochondrial DNA (mtDNA); oxidative damage; repair mechanisms fructose-rich diet; mitochondrial biogenesis; mitochondrial DNA (mtDNA); oxidative damage; repair mechanisms

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Cioffi, F.; Senese, R.; Lasala, P.; Ziello, A.; Mazzoli, A.; Crescenzo, R.; Liverini, G.; Lanni, A.; Goglia, F.; Iossa, S. Fructose-Rich Diet Affects Mitochondrial DNA Damage and Repair in Rats. Nutrients 2017, 9, 323.

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