Dietary Animal Plasma Proteins Improve the Intestinal Immune Response in Senescent Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals and Diets
2.2. Experimental Design
2.3. MLN Cell Isolation
2.4. PP Cell Isolation
2.5. Cell Staining
2.6. Serum Cytokine Determination
2.7. Real-Time Polimerase Chain Reaction
2.8. Statistical Analysis
3. Results
3.1. Characterization of GALT in SAM Strains
3.1.1. MLN Populations in SAM Strains
3.1.2. PP Populations in SAM Strains
3.1.3. Pro-Inflammatory Cytokines in SAM Strains
3.2. Effect of SDP on Senescent Mice
3.2.1. Effect of SDP on Systemic Variables
3.2.2. Effect of SDP on MLN Populations in Senescent Mice
3.2.3. Effect of SDP on PP Populations in Senescent Mice
3.2.4. Effect of SDP on Cytokine Expression in Senescent Mice
4. Discussion
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Ingredients | Control Diet | SDP 1 Diet |
---|---|---|
g/kg | ||
SDP | - | 80.0 |
Dried skim milk | 530.7 | 340.5 |
Corn starch | 199.3 | 308.8 |
Sucrose | 94.5 | 94.5 |
Soybean oil | 70 | 70 |
Cellulose | 50 | 50 |
AIN-93-G-MX (94046) 2 | 35 | 35 |
AIN-93 VX (94047) 2 | 15 | 15 |
DL-Methionine | 2.5 | 3.2 |
Choline bitartrate | 3 | 3 |
Primer | Forward (5′-3′) | Reverse (5′-3′) | Fragment Size | Accession Number |
---|---|---|---|---|
Gusβ | CCGATTATCCAGAGCGAGTATG | CTCAGCGGTGACTGGTTCG | 197 bp | NM_010368.1 |
Il-6 | ACCAGAGGAAATTTTCAATAGGC | TGATGCACTTGCAGAAAACA | 109 bp | NM_031168.1 |
Il-10 | GGCGCTGTCATCGATTTCTCCCC | TGGCCTTGTAGACACCTTGGTCTT | 102 bp | NM_010548.2 |
Inf-γ | CCTTCTTCAGCAACAGCAAGGCG | CTTGGCGCTGGACCTGTGGG | 87 bp | NM_008337.4 |
Tnf-α | CCACCACGCTCTTCTGTCTAC | AGGGTCTGGGCCATAGAACT | 103 bp | NM_013693.2 |
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Miró, L.; Garcia-Just, A.; Amat, C.; Polo, J.; Moretó, M.; Pérez-Bosque, A. Dietary Animal Plasma Proteins Improve the Intestinal Immune Response in Senescent Mice. Nutrients 2017, 9, 1346. https://doi.org/10.3390/nu9121346
Miró L, Garcia-Just A, Amat C, Polo J, Moretó M, Pérez-Bosque A. Dietary Animal Plasma Proteins Improve the Intestinal Immune Response in Senescent Mice. Nutrients. 2017; 9(12):1346. https://doi.org/10.3390/nu9121346
Chicago/Turabian StyleMiró, Lluïsa, Alba Garcia-Just, Concepció Amat, Javier Polo, Miquel Moretó, and Anna Pérez-Bosque. 2017. "Dietary Animal Plasma Proteins Improve the Intestinal Immune Response in Senescent Mice" Nutrients 9, no. 12: 1346. https://doi.org/10.3390/nu9121346