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Nutrients
Volume 9
Issue 12
10.3390/nu9121329
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Article

Meta-Analysis of Fecal Microbiota and Metabolites in Experimental Colitic Mice during the Inflammatory and Healing Phases

by
Toshifumi Osaka
1,2,†,
Eri Moriyama
2,†,
Shunichi Arai
2,
Yasuhiro Date
3,4,
Junji Yagi
1,
Jun Kikuchi
3,4,5 and
Satoshi Tsuneda
2,*
1
Department of Microbiology and Immunology, Tokyo Women’s Medical University, 8-1, Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan
2
Department of Life Science and Medical Bioscience, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan
3
RIKEN Center for Sustainable Resource Science, 1-7-22 Suehirocho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
4
Graduate School of Medical Life Science, Yokohama City University, 1-7-29 Suehirocho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
5
Graduate School of Bioagricultural Sciences, Nagoya University, 1 Furo-cho, Chikusa-ku, Nagoya, Aichi 464-0810, Japan
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Nutrients 2017, 9(12), 1329; https://doi.org/10.3390/nu9121329
Received: 28 September 2017 / Revised: 28 November 2017 / Accepted: 3 December 2017 / Published: 6 December 2017
(This article belongs to the Special Issue Prebiotics and Probiotics)
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Abstract

The imbalance of gut microbiota is known to be associated with inflammatory bowel disease, but it remains unknown whether dysbiosis is a cause or consequence of chronic gut inflammation. In order to investigate the effects of gut inflammation on microbiota and metabolome, the sequential changes in gut microbiota and metabolites from the onset of colitis to the recovery in dextran sulfate sodium-induced colitic mice were characterized by using meta 16S rRNA sequencing and proton nuclear magnetic resonance (1H-NMR) analysis. Mice in the colitis progression phase showed the transient expansions of two bacterial families including Bacteroidaceae and Enterobacteriaceae and the depletion of major gut commensal bacteria belonging to the uncultured Bacteroidales family S24-7, Rikenellaceae, Lachnospiraceae, and Ruminococcaceae. After the initiation of the recovery, commensal Lactobacillus members promptly predominated in gut while other normally abundant bacteria excluding the Erysipelotrichaceae remained diminished. Furthermore, 1H-NMR analysis revealed characteristic fluctuations in fecal levels of organic acids (lactate and succinate) associated with the disease states. In conclusion, acute intestinal inflammation is a perturbation factor of gut microbiota but alters the intestinal environments suitable for Lactobacillus members. View Full-Text
Keywords: gut microbiota; dysbiosis; inflammatory bowel disease; metabolome; meta 16S rRNA analysis; 1H-NMR analysis; experimental colitic mice; Lactobacillus gut microbiota; dysbiosis; inflammatory bowel disease; metabolome; meta 16S rRNA analysis; 1H-NMR analysis; experimental colitic mice; Lactobacillus
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MDPI and ACS Style

Osaka, T.; Moriyama, E.; Arai, S.; Date, Y.; Yagi, J.; Kikuchi, J.; Tsuneda, S. Meta-Analysis of Fecal Microbiota and Metabolites in Experimental Colitic Mice during the Inflammatory and Healing Phases. Nutrients 2017, 9, 1329. https://doi.org/10.3390/nu9121329

AMA Style

Osaka T, Moriyama E, Arai S, Date Y, Yagi J, Kikuchi J, Tsuneda S. Meta-Analysis of Fecal Microbiota and Metabolites in Experimental Colitic Mice during the Inflammatory and Healing Phases. Nutrients. 2017; 9(12):1329. https://doi.org/10.3390/nu9121329

Chicago/Turabian Style

Osaka, Toshifumi, Eri Moriyama, Shunichi Arai, Yasuhiro Date, Junji Yagi, Jun Kikuchi, and Satoshi Tsuneda. 2017. "Meta-Analysis of Fecal Microbiota and Metabolites in Experimental Colitic Mice during the Inflammatory and Healing Phases" Nutrients 9, no. 12: 1329. https://doi.org/10.3390/nu9121329

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