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N-3 Polyunsaturated Fatty Acids Improve Liver Lipid Oxidation-Related Enzyme Levels and Increased the Peroxisome Proliferator-Activated Receptor α Expression Level in Mice Subjected to Hemorrhagic Shock/Resuscitation

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Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Zhongshan East Road 305, Nanjing 210002, China
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Department of General Surgery, Jinling Hospital, Clinical College of Southern Medical University, Zhongshan East Road 305, Nanjing 210002, China
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Author to whom correspondence should be addressed.
Nutrients 2016, 8(4), 237; https://doi.org/10.3390/nu8040237
Received: 16 February 2016 / Revised: 9 April 2016 / Accepted: 15 April 2016 / Published: 22 April 2016
Appropriate metabolic interventions after hemorrhagic shock/resuscitation injury have not yet been identified. We aimed to examine the effects of fish oil on lipid metabolic intervention after hemorrhagic shock/resuscitation. Firstly, 48 C57BL/6 mice were assigned to six groups (n = 8 per group). The sham group did not undergo surgery, while mice in the remaining groups were sacrificed 1–5 days after hemorrhagic shock/resuscitation. In the second part, mice were treated with saline or fish oil (n = 8 per group) five days after injury. We determined serum triglyceride levels and liver tissues were collected and prepared for qRT-PCR or Western blot analysis. We found that triglyceride levels were increased five days after hemorrhagic shock/resuscitation, but decreased after addition of fish oil. After injury, the protein and gene expression of carnitine palmitoyltransferase 1A, fatty acid transport protein 1, and peroxisome proliferator-activated receptor-α decreased significantly in liver tissue. In contrast, after treatment with fish oil, the expression levels of these targets increased compared with those in the saline group. The present results suggest n-3 polyunsaturated fatty acids could improve lipid oxidation-related enzymes in liver subjected to hemorrhagic shock/resuscitation. This function is possibly accomplished through activating the peroxisome proliferator-activated receptor-α pathway. View Full-Text
Keywords: hemorrhagic shock; peroxisome proliferator-activated receptor; fatty acid oxidation; fish oil hemorrhagic shock; peroxisome proliferator-activated receptor; fatty acid oxidation; fish oil
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Zhang, L.; Tian, F.; Gao, X.; Wang, X.; Wu, C.; Li, N.; Li, J. N-3 Polyunsaturated Fatty Acids Improve Liver Lipid Oxidation-Related Enzyme Levels and Increased the Peroxisome Proliferator-Activated Receptor α Expression Level in Mice Subjected to Hemorrhagic Shock/Resuscitation. Nutrients 2016, 8, 237.

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